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首页> 外文期刊>American Journal of Physiology >Probiotics potentiate IL-6 production in IL-1beta-treated Caco-2 cells through a heat shock-dependent mechanism.
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Probiotics potentiate IL-6 production in IL-1beta-treated Caco-2 cells through a heat shock-dependent mechanism.

机译：益生菌通过热休克依赖性机制增强IL-1beta处理的Caco-2细胞中IL-6的产生。

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摘要

IL-6 may exert anti-inflammatory and protective effects in intestinal mucosa and enterocytes. The influence of probiotics on mucosal and enterocyte IL-6 production is not known. We tested the hypothesis that the probiotic bacteria Lactobacillus paracasei and Lactobacillus plantarum regulate IL-6 production in intestinal epithelial cells. Cultured Caco-2 cells were treated with 1 ng/ml of IL-1beta in the absence or presence of different concentrations of L. paracasei or L. plantarum followed by measurement of IL-6 production. The role of heat shock response was examined by determining the expression of heat shock protein 70 (hsp70) and hsp27, by downregulating their expression with small interfering RNA (siRNA), or by treating cells with quercetin. Treatment of the Caco-2 cells with IL-1beta resulted in increased IL-6 production, confirming previous reports from this laboratory. Probiotics alone did not influence IL-6 production, but the addition of probitoics to IL-1beta-treated cells resulted in a substantial augmentation of IL-6 production. Treatment of the Caco-2 cells with live L. paracasei increased cellular levels of hsp70 and hsp27 and the potentiating effect on IL-6 production was inhibited by quercetin and by hsp70 or hsp27 siRNA. Results suggest that probiotics may enhance IL-6 production in enterocytes subjected to an inflammatory stimulus and that this effect may, at least in part, be heat shock dependent.
机译：IL-6可能在肠粘膜和肠上皮细胞中发挥抗炎和保护作用。益生菌对粘膜和肠细胞IL-6产生的影响尚不清楚。我们测试了益生菌副干酪乳杆菌和植物乳杆菌调节肠上皮细胞中IL-6产生的假说。在不存在或存在不同浓度的副干酪乳杆菌或植物乳杆菌的情况下，用1 ng / ml IL-1beta处理培养的Caco-2细胞，然后测量IL-6的产生。通过确定热激蛋白70（hsp70）和hsp27的表达，通过用小干扰RNA（siRNA）下调它们的表达或用槲皮素处理细胞来检查热激反应的作用。用IL-1β处理Caco-2细胞导致IL-6产生增加，证实了该实验室先前的报道。单独的益生菌并不会影响IL-6的产生，但是向IL-1beta处理的细胞中添加probitoics会导致IL-6产生的大量增加。用活副干酪乳杆菌处理Caco-2细胞可增加hsp70和hsp27的细胞水平，并且槲皮素和hsp70或hsp27 siRNA抑制了对IL-6产生的增强作用。结果表明，益生菌可以增强受到炎性刺激的肠细胞中IL-6的产生，并且这种作用可能至少部分取决于热休克。

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