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首页> 外文期刊>American Journal of Physiology >Propagation of calcium waves along endothelium of hamster feed arteries.
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Propagation of calcium waves along endothelium of hamster feed arteries.

机译：钙波沿仓鼠饲料动脉内皮的传播。

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摘要

An increase in tissue blood flow requires relaxation of smooth muscle cells along entire branches of the resistance vasculature. Whereas the spread of hyperpolarization along the endothelium can coordinate smooth muscle cell relaxation, complementary signaling events have been implicated in the conduction of vasodilation. We tested the hypothesis that Ca(2+) waves propagate from cell to cell along the endothelium of feed arteries exhibiting spontaneous vasomotor tone. Feed arteries of the hamster retractor muscle were isolated, pressurized to 75 mmHg at 37 degrees C, and developed myogenic tone spontaneously. Smooth muscle cells and endothelial cells were loaded with the Ca(2+) indicator Fluo-4. An acetylcholine stimulus was delivered locally using microiontophoresis (1-microm pipette tip, 1 microA, 1 s), and Ca(2+) signaling within and along respective cell layers was determined using laser-scanning confocal microscopy. Acetylcholine triggered an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) of endothelial cells at the site of stimulation that preceded two distinct events: 1) a rapid synchronous decrease in smooth muscle [Ca(2+)](i) along the entire vessel and 2) an ensuing Ca(2+) wave that propagated bidirectionally along the endothelium at approximately 111 microm/s for distances exceeding 1 mm. Maximal dilation of vessels with either nifedipine (1 microM) or sodium nitroprusside (SNP, 100 microM) reduced the distance that Ca(2+) waves traveled to approximately 300 microm (P < 0.05). Thus Ca(2+) waves propagate along the endothelium of resistance vessels with vasomotor tone, and this signaling pathway is compromised during maximal dilation with nifedipine or SNP.
机译：组织血流量的增加要求平滑肌细胞沿阻力脉管系统的整个分支松弛。尽管超极化沿内皮细胞的扩散可以协调平滑肌细胞的松弛，但是在血管舒张的传导中涉及了互补的信号传导事件。我们测试了Ca（2+）波沿展示自发性血管舒缩张力的饲料动脉内皮细胞从一个细胞传播到另一个细胞的假说。分离仓鼠牵开肌的饲料动脉，在37摄氏度下加压至75 mmHg，并自发形成肌源性肌张力。平滑肌细胞和内皮细胞装有Ca（2+）指示剂Fluo-4。使用微离子电渗疗法（1微米移液器吸头，1 microA，1 s）局部递送乙酰胆碱刺激，并使用激光扫描共聚焦显微镜确定各个细胞层内和沿各个细胞层的Ca（2+）信号传导。乙酰胆碱在发生两个明显事件之前的刺激部位触发了内皮细胞的细胞内Ca（2+）浓度（[Ca（2 +）]（i））的增加：1）平滑肌[Ca（ 2 +）]（i）沿着整个血管，以及2）随后的Ca（2+）波以大约111 microm / s的速度沿内皮双向传播，距离超过1 mm。硝苯地平（1 microM）或硝普钠（SNP，100 microM）的血管最大扩张减少了Ca（2+）波传播至大约300 microm的距离（P <0.05）。因此，Ca（2+）波沿着具有血管舒缩张力的阻力血管的内皮细胞传播，并且该信号通路在硝苯地平或SNP的最大扩张过程中受到损害。

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