• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern.
【24h】

Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern.

机译：饮食中蛋氨酸的限制可改善结肠紧密连接屏障的功能并改变claudin的表达方式。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The beneficial effects of caloric restriction in increasing longevity and forestalling age-related diseases are well known. Dietary restriction of methionine also renders similar benefits. We recently showed in a renal epithelial cell culture system that reduction of culture medium methionine by 80% resulted in altered tight junctional (TJ) claudin composition and also improved epithelial barrier function (51). In the current study, we examined the effect of dietary restriction of methionine on TJ barrier function in rat gastrointestinal tissue to see whether this phenomenon also holds true in a tissue model and for a different epithelial cell type. After 28 days on methionine-restricted (MR) diet, rats showed small but significant reductions in the plasma and (intracellular) colonocyte levels of methionine. Colon mucosal sheets from rats on the MR diet showed increased transepithelial electrical resistance with concomitant decrease in paracellular diffusion of (14)C-D-mannitol, suggesting improved barrier function relative to rats on control diet. This improved barrier function could not be explained by changes in colon crypt length or frequency. Neither was the colonocyte mitotic index nor the apoptotic frequency altered significantly. However, TJ composition/structure was being altered by the MR diet. RT-PCR and Western blot analysis showed an increase in the abundance of claudin-3 and an apparent change in the posttranslational modification of occludin, data reinforcing a paracellular barrier alteration. Overall, our data suggest that reduction in dietary intake of methionine results in improved epithelial barrier function by inducing altered TJ protein composition.
机译：热量限制在延长寿命和预防与年龄有关的疾病中的有益作用是众所周知的。饮食中蛋氨酸的限制也有类似的好处。我们最近在肾脏上皮细胞培养系统中发现，将蛋氨酸培养基减少80％会导致紧密连接（TJ）claudin组成发生变化，并改善上皮屏障功能（51）。在当前的研究中，我们研究了饮食中蛋氨酸的限制对大鼠胃肠道组织中TJ屏障功能的影响，以查看这种现象在组织模型和不同的上皮细胞类型中是否也适用。蛋氨酸限制（MR）饮食28天后，大鼠血浆中蛋氨酸的血浆和（细胞内）结肠细胞水平出现少量但显着的降低。 MR饮食的大鼠结肠粘膜表层显示跨上皮电阻增加，同时（14）C-D-甘露醇的细胞旁扩散减少，提示相对于饮食控制的大鼠，屏障功能得到改善。结肠隐窝长度或频率的改变不能解释这种改善的屏障功能。结肠细胞的有丝分裂指数和凋亡频率都没有明显改变。然而，MR饮食改变了TJ的组成/结构。 RT-PCR和蛋白质印迹分析显示claudin-3的丰度增加，而occludin的翻译后修饰发生了明显变化，数据加强了旁细胞屏障的改变。总体而言，我们的数据表明，饮食中蛋氨酸摄入的减少会通过诱导TJ蛋白组成的改变而改善上皮屏障功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号