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首页> 外文期刊>American Journal of Physiology >Gender influences the response to experimental silica-induced lung fibrosis in mice.
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Gender influences the response to experimental silica-induced lung fibrosis in mice.

机译：性别影响小鼠对实验性二氧化硅诱导的肺纤维化的反应。

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摘要

Accumulating evidence suggests that gender can have a profound effect on incidence and severity of a variety of pulmonary diseases. To address the influence of gender on the development of silica-induced pulmonary fibrosis, we instilled 0.2 g/kg silica into male and female C57BL/6 mice and examined the fibrotic and inflammatory response at 14 days postexposure. Both silica-exposed male and female mice had significant increases in total lung hydroxyproline compared with saline controls. However, silica-exposed female mice had significantly less total lung hydroxyproline than silica-exposed male mice. This observation was confirmed by color thresholding image analysis. Interestingly, silica-exposed female mice had significantly more inflammatory cells, the majority of which were macrophages, as well as higher levels of the macrophage-specific chemokines MCP-1 and CCL9 in whole lung lavage compared with silica-exposed male mice. We also show that at baseline, estrogen receptor alpha (ERalpha) mRNA expression is lower in female mice than in males and that ERalpha mRNA expression is decreased by silica exposure. Finally, we show that the response of ovariectomized female mice to silica instillation is similar to that of male mice. These observations together show that gender influences the lung response to silica.
机译：越来越多的证据表明，性别可以对多种肺部疾病的发生率和严重程度产生深远影响。为了解决性别对二氧化硅诱发的肺纤维化发展的影响，我们向雄性和雌性C57BL / 6小鼠滴注了0.2 g / kg二氧化硅，并在暴露后14天检查了纤维化和炎症反应。与盐水对照组相比，暴露于二氧化硅的雄性和雌性小鼠的总肺羟脯氨酸含量均显着增加。但是，暴露于二氧化硅的雌性小鼠的总肺羟脯氨酸明显少于暴露于二氧化硅的雄性小鼠。该观察结果通过颜色阈值图像分析得到证实。有趣的是，与暴露于二氧化硅的雄性小鼠相比，暴露于二氧化硅的雌性小鼠具有明显更多的炎性细胞，其中大多数是巨噬细胞，而且整个肺灌洗液中的巨噬细胞特异性趋化因子MCP-1和CCL9的水平更高。我们还显示，在基线时，雌性小鼠的雌激素受体α（ERalpha）mRNA表达低于雄性，并且暴露于二氧化硅的雌激素受体α（ERalpha）mRNA表达降低。最后，我们表明去卵巢的雌性小鼠对硅胶滴注的反应与雄性小鼠相似。这些观察结果共同表明性别影响肺对二氧化硅的反应。

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