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首页> 外文期刊>American Journal of Physiology >Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation.
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Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation.

机译：小鼠血管周围和褐色脂肪组织的相似性及其对饮食诱发的炎症的抵抗力。

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Thoracic perivascular adipose tissue (PVAT) is a unique adipose depot that likely influences vascular function and susceptibility to pathogenesis in obesity and the metabolic syndrome. Surprisingly, PVAT has been reported to share characteristics of both brown and white adipose, but a detailed direct comparison to interscapular brown adipose tissue (BAT) has not been performed. Here we show by full genome DNA microarray analysis that global gene expression profiles of PVAT are virtually identical to BAT, with equally high expression of Ucp-1, Cidea, and other genes known to be uniquely or very highly expressed in BAT. PVAT and BAT also displayed nearly identical phenotypes upon immunohistochemical analysis, and electron microscopy confirmed that PVAT contained multilocular lipid droplets and abundant mitochondria. Compared with white adipose tissue (WAT), PVAT and BAT from C57BL6/J mice fed a high-fat diet for 13 wk had markedly lower expression of immune cell-enriched mRNAs, suggesting resistance to obesity-induced inflammation. Indeed, staining of BAT and PVAT for macrophage markers (F4/80 and CD68) in obese mice showed virtually no macrophage infiltration, and FACS analysis of BAT confirmed the presence of very few CD11b(+)/CD11c(+) macrophages in BAT (1.0%) compared with WAT (31%). In summary, murine PVAT from the thoracic aorta is virtually identical to interscapular BAT, is resistant to diet-induced macrophage infiltration, and thus may play an important role in protecting the vascular bed from inflammatory stress.
机译：胸腔血管周围脂肪组织（PVAT）是独特的脂肪库，它可能影响肥胖症和代谢综合征患者的血管功能和发病机理的敏感性。出人意料的是，据报道PVAT具有棕色和白色脂肪的共同特征，但尚未进行与肩inter间棕色脂肪组织（BAT）的详细直接比较。在这里，我们通过全基因组DNA微阵列分析显示，PVAT的全球基因表达谱与BAT几乎相同，Ucp-1，Cidea以及其他已知在BAT中独特或非常高表达的基因的表达同样高。通过免疫组织化学分析，PVAT和BAT也显示出几乎相同的表型，电子显微镜证实PVAT包含多眼脂质滴和丰富的线粒体。与白色脂肪组织（WAT）相比，以高脂饮食喂养13周的C57BL6 / J小鼠的PVAT和BAT的免疫细胞富集的mRNA表达明显降低，表明对肥胖引起的炎症具有​​抵抗力。确实，肥胖小鼠巨噬细胞标记物（F4 / 80和CD68）的BAT和PVAT染色几乎没有显示巨噬细胞浸润，并且BAT的FACS分析证实了BAT中几乎没有CD11b（+）/ CD11c（+）巨噬细胞的存在（ 1.0％），而WAT（31％）。总之，来自胸主动脉的鼠PVAT实际上与肩inter间BAT相同，对饮食诱导的巨噬细胞浸润具有抵抗力，因此可能在保护血管床免受炎性应激中起重要作用。

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