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首页> 外文期刊>American Journal of Physiology >Heterogeneity in histone 2B-green fluorescent protein-retaifiing-putative small intestinal stem cells at cell position 4 and their absence in the colon
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Heterogeneity in histone 2B-green fluorescent protein-retaifiing-putative small intestinal stem cells at cell position 4 and their absence in the colon

机译：组蛋白2B-绿色荧光蛋白修复性小肠干细胞在细胞位置4的异质性及其在结肠中的缺失

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摘要

Stem cells have been identified in two locations in small intestinal crypts; those intercalated between Paneth cells and another population (which retains DNA label) are located above the Paneth cell zone, at cell position 4. Because of disadvantages associated with the use of DNA label, doxycycline-induced transient transgenic expression of histone 2B (H2B)-green fluorescent protein (GFP) Vas investigated. H2B-GFP-retaining putative stem cells were consistently seen, with a peak at cell position 4, over chase periods of up to 112 days. After a 28-day chase, a subpopulation of the H2B-GFP-retaining cells was cycling, but the slow cycling status of the majority was illustrated by lack of expression of pHistone H3 and Ki67. Although some H2B-GFP-retaining cells were sensitive to low-dose radiation, the majority was resistant to low- and high-dose radiation-induced cell death, and a proportion of the surviving cells proliferated during subsequent epithelial regeneration. Long-term retention of H2B-GFP in a subpopulation of small intestinal Paneth cells was also seen, implying that they are long lived. In contrast to the small intestine, H2B-GFP-retaining epithelial cells were not seen in the colon from 28-day chase onward. This implies important differences in stem cell function between these two regions of the gastrointestinal tract, which may have implications for region-specific susceptibility to diseases (such as cancer and ulcerative colitis), in which epithelial stem cells and their progeny are involved.
机译：在小肠隐窝的两个位置已鉴定出干细胞。那些介于Paneth细胞和另一个种群（保留DNA标记）之间的分子位于Paneth细胞区的上方，细胞位置4。由于使用DNA标记的缺点，强力霉素诱导组蛋白2B（H2B）的瞬时转基因表达研究了绿色荧光蛋白（GFP）。持续观察到H2B-GFP保留的干细胞，在细胞位置4出现一个峰值，历时长达112天。经过28天的追踪，H2B-GFP保留细胞的亚群开始循环，但是大多数的缓慢循环状态可以通过pHistone H3和Ki67的缺乏表达来说明。尽管一些保留H2B-GFP的细胞对低剂量辐射敏感，但大多数细胞对低剂量和高剂量辐射诱导的细胞死亡具有抵抗力，并且一部分存活细胞在随后的上皮再生过程中增殖。还观察到H2B-GFP在小肠Paneth细胞亚群中的长期保留，这表明它们是长寿的。与小肠相反，从28天开始，在结肠中未见到H2B-GFP保留的上皮细胞。这暗示着胃肠道的这两个区域之间干细胞功能的重要差异，这可能对涉及上皮干细胞及其后代的疾病（例如癌症和溃疡性结肠炎）的区域特异性易感性产生影响。

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