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首页> 外文期刊>Analytica chimica acta >Amperometric enzyme electrodes of glucose and lactate based on poly(diallyldimethylammonium)-alginate-metal ion-enzyme biocomposites
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Amperometric enzyme electrodes of glucose and lactate based on poly(diallyldimethylammonium)-alginate-metal ion-enzyme biocomposites

机译：基于聚（二烯丙基二甲基铵）-藻酸盐-金属离子酶生物复合材料的葡萄糖和乳酸的安培酶电极

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摘要

Sodium alginate (AlgNa) and poly(diallyldimethylammonium chloride) (PDDA) were mixed to obtain an interpenetrating polymer composite via electrostatic interaction and then cast on an Au electrode surface, followed by incorporation of metal ions (e.g. Fe~(3+) or Ca~(2+), to form AlgFe or AlgCa hydrogel) and glucose oxidase (GOx) (or lactate oxidase (LOx)), to prepare amperometric enzyme electrodes. The interactions of PDDA, Alg, and Fe~(3+) are studied by visual inspection as well as microscopic and electrochemical methods. Under optimized conditions, the PDDA-AlgFe-enzyme/Au and PDDA-AlgCa-enzyme/Au electrodes can give good analytical performance (e.g. nM-scale limit of detection of glucose or lactate, and sensitivities > 50 μAcm~(-2) mM~(-1)) in the first-generation biosensing mode, which are better than the reported analogs using typical polysaccharide biopolymers as enzyme-immobilization matrices. The enzyme electrodes also worked well in the second-generation biosensing mode in the coexistence of p-benzoquione or ferrocene monocarboxylic acid artificial mediator. Biofuel cells (BFCs) with the enzyme electrodes as the bioanodes and glucose (or lactate) as the biofuel were also fabricated with satisfactory results. The proposed protocols for preparation of high performance Alg-based biocomposites may find wide applications in bioanalysis.
机译：将藻酸钠（AlgNa）和聚二烯丙基二甲基氯化铵（PDDA）混合，通过静电相互作用获得互穿聚合物复合材料，然后浇铸在Au电极表面，然后掺入金属离子（例如Fe〜（3+）或Ca 〜（2+），形成AlgFe或AlgCa水凝胶）和葡萄糖氧化酶（GOx）（或乳酸氧化酶（LOx）），以制备电流型酶电极。通过目测以及微观和电化学方法研究了PDDA，Alg和Fe〜（3+）的相互作用。在最佳条件下，PDDA-AlgFe酶/ Au和PDDA-AlgCa酶/ Au电极可提供良好的分析性能（例如，葡萄糖或乳酸盐的nM级检测限，灵敏度> 50μAcm〜（-2）mM） 〜（-1））在第一代生物传感模式下，比使用典型的多糖生物聚合物作为酶固定基质的报道类似物更好。酶电极在第二代生物传感模式下与对苯并醌或二茂铁一元羧酸人工介体共存时也能很好地工作。酶电极作为生物阳极，葡萄糖（或乳酸）作为生物燃料的生物燃料电池（BFC）也得到了满意的结果。提议的用于制备基于Alg的高性能生物复合材料的方案可能会在生物分析中获得广泛的应用。

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