首页> 外文期刊>Analytica chimica acta >Planar monolithic porous polymer layers functionalized with gold nanoparticles as large-area substrates for sensitive surface-enhanced Raman scattering sensing of bacteria
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Planar monolithic porous polymer layers functionalized with gold nanoparticles as large-area substrates for sensitive surface-enhanced Raman scattering sensing of bacteria

机译:用金纳米颗粒功能化的平面整体多孔聚合物层,作为大面积基质,用于细菌的敏感表面增强拉曼散射传感

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摘要

For the first time, large-area surface-enhanced Raman scattering sensing active substrates using porous polymer monolithic layers have been successfully prepared. Our approach includes a simple photo-initiated polymerization process using glycidyl methacrylate and ethylene dimethacrylate in a glass mold, followed by a chemical reaction of the epoxy functionalities leading to thiols, and the attachment of preformed gold nanoparticles. We demonstrated that this very simple process produced uniform and reproducible large area surfaces that significantly enhance sensitivity of Raman spectroscopy. Experiments were also carried out that confirmed preferential adsorption of living bacteria Escherichia coli from a very dilute solution on the surface of the monolithic layer, and immediate detection of the captured microorganisms using the SERS spectrum. (C) 2015 Elsevier B.V. All rights reserved.
机译:首次成功地制备了使用多孔聚合物整体层的大面积表面增强拉曼散射传感有源基板。我们的方法包括在玻璃模具中使用甲基丙烯酸缩水甘油酯和二甲基丙烯酸乙二酯进行简单的光引发聚合过程,然后进行环氧官能团的化学反应,形成硫醇,并附着预先形成的金纳米颗粒。我们证明了这一非常简单的过程可产生均匀且可重现的大面积表面,从而大大提高了拉曼光谱的灵敏度。还进行了实验,证实了活菌大肠杆菌从非常稀溶液中优先吸附在整体层表面上,并使用SERS光谱立即检测了捕获的微生物。 (C)2015 Elsevier B.V.保留所有权利。

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