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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Is Relative Quantification Dispensable for the Measurement of MicroRNAs as Serum Biomarkers in Germ Cell Tumors?
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Is Relative Quantification Dispensable for the Measurement of MicroRNAs as Serum Biomarkers in Germ Cell Tumors?

机译：对于生殖细胞肿瘤中作为血清生物标志物的microRNA的测量，是否需要相对定量？

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摘要

Background: Classical biomarkers alpha-fetoprotein, beta-human chorionic gonadotropin and lactate dehydrogenase (AFP, bHCG and LDH) are elevated in only 60% of all testicular germ cell tumor (TGCT) patients. microRNAs (miRNAs) are a novel class of useful biomarkers in cancer and miRNAs of the miR-371-3 cluster were proven to be valuable markers for TGCT patients. Materials and Methods: We compared the Ct and Delta Ct values of miR-371-3 by real time PCR (qPCR) with and without 18S rRNA for normalization. Expression of miR-371a-3p, miR-372 and miR-373-3p was measured in 25 TGCTs, 4 non-TGCTs and 17 age-matched male controls. Results: A highly positive correlation between Ct and Delta Ct values was found in all samples. The highest correlation was found for miR-371a-3p (R2: 0.956). Conclusion: Results show that qPCR can be used without endogenous control for analyzing miR-371-3 in the serum of patients with testicular cancer and male controls if the technical procedure is performed under controlled conditions.
机译：背景：经典的生物标志物甲胎蛋白，β-人绒毛膜促性腺激素和乳酸脱氢酶（AFP，bHCG和LDH）仅在60％的睾丸生殖细胞肿瘤（TGCT）患者中升高。 microRNA（miRNA）是一类新的有用的癌症生物标志物，miR-371-3簇的miRNA被证明是TGCT患者的有价值的标志物。材料和方法：我们通过实时PCR（qPCR）比较有无18S rRNA的miR-371-3的Ct和Delta Ct值进行标准化。在25个TGCT，4个非TGCT和17个年龄匹配的男性对照中测量了miR-371a-3p，miR-372和miR-373-3p的表达。结果：在所有样品中Ct和Delta Ct值之间都存在高度正相关。发现miR-371a-3p的相关性最高（R2：0.956）。结论：结果表明，如果在受控条件下进行技术操作，则qPCR可在没有内源性对照的情况下用于分析睾丸癌患者和男性对照患者血清中的miR-371-3。

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