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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer
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Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer

机译：大肠癌中MnSOD和GPX1基因表达及血清水平的测定

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Background/Aim: Oxidative stress plays a role on the development of colorectal cancer. Manganese superoxide disnzutase (MnSOD) and glutathione peroxidase I (GPXI) are crucial in regulating oxidative balance and its stabilization. Possible mechanisms of action of these enzymes in various types of cancers require further investigation. We aimed to determine expression levels of these genes and their effects on protein levels in serum of patients with colorectal cancer. Materials and Methods: Expression levels of genes were determined using Real Time-Polymerase chain reaction in 35 patients with colorectal cancer. We used enzyme-linked immunosorbent assay to determine MnSOD and GPXI levels. Results: We found significant differences in GPXI expression between tumor and normal tissues, with a 2-fold decrease in tumor tissues (p<0.05). However, although no significant difference was found between the expression of MnSOD gene in tumor and that in normal tissues, there was a 1.13-fold change in expression. We observed no relationship between expressions of either gene and their levels in serum. Conclusion: The GPXI gene may play a critical role in the development of colorectal cancer.
机译：背景/目的：氧化应激在结直肠癌的发展中起作用。锰超氧化物歧化酶（MnSOD）和谷胱甘肽过氧化物酶I（GPXI）在调节氧化平衡及其稳定中至关重要。这些酶在各种类型的癌症中可能的作用机制需要进一步研究。我们旨在确定这些基因的表达水平及其对大肠癌患者血清中蛋白质水平的影响。材料和方法：使用实时聚合酶链反应测定35例大肠癌患者的基因表达水平。我们使用酶联免疫吸附测定法测定MnSOD和GPXI水平。结果：我们发现肿瘤组织和正常组织之间GPXI表达有显着差异，肿瘤组织下降了2倍（p <0.05）。然而，尽管在肿瘤组织和正常组织中的MnSOD基因的表达之间没有发现显着差异，但是表达上有1.13倍的变化。我们观察到任一基因的表达与其血清水平之间没有关系。结论：GPXI基因可能在结直肠癌的发生中起关键作用。

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