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首页> 外文期刊>Antimicrobial agents and chemotherapy. >Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli
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Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli

机译：鲍曼不动杆菌的AdeABC和AdeIJK外排系统与大肠杆菌AcrAB-TolC系统的性能比较

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摘要

Acinetobacter baumannii contains RND-family efflux systems AdeABC and AdeIJK, which pump out a wide range of antimicrobial compounds, as judged from the MIC changes occurring upon deletion of the responsible genes. However, these studies may miss changes because of the high backgrounds generated by the remaining pumps and by β-lactamases, and it is unclear how the activities of these pumps compare quantitatively with those of the well-studied AcrAB-TolC system of Escherichia coli. We expressed adeABC and adeIJK of A. baumannii, as well as E. coli acrAB, in an E. coli host from which acrAB was deleted. The A. baumannii pumps were functional in E. coli, and the MIC changes that were observed largely confirmed the substrate range already reported, with important differences. Thus, the AdeABC system pumped out all β-lactams, an activity that was often missed in deletion studies. When the expression level of the pump genes was adjusted to a similar level for a comparison with AcrAB-TolC, we found that both A. baumannii efflux systems pumped out a wide range of compounds, but AdeABC was less effective than AcrAB-TolC in the extrusion of lipophilic β-lactams, novobiocin, and ethidium bromide, although it was more effective at tetracycline efflux. AdeIJK was remarkably more effective than a similar level of AcrAB-TolC in the efflux of β-lactams, novobiocin, and ethidium bromide, although it was less so in the efflux of erythromycin. These results thus allow us to compare these efflux systems on a quantitative basis, if we can assume that the heterologous systems are fully functional in the E. coli host.
机译：鲍曼不动杆菌包含RND家族外排系统AdeABC和AdeIJK，它们从广泛的抗菌化合物中抽出，这是根据负责基因缺失后发生的MIC变化判断的。然而，由于剩余泵和β-内酰胺酶产生的高本底，这些研究可能会错过变化，目前尚不清楚这些泵的活性如何与经过充分研究的大肠杆菌AcrAB-TolC系统进行定量比较。我们在删除了acrAB的大肠杆菌宿主中表达了鲍曼不动杆菌的adeABC和adeIJK，以及大肠杆菌acrAB。鲍曼不动杆菌泵在大肠杆菌中起作用，观察到的MIC变化在很大程度上证实了已报道的底物范围，但有重要区别。因此，AdeABC系统抽出了所有β-内酰胺，这种活性在缺失研究中经常被忽略。当将泵浦基因的表达水平调整到与AcrAB-TolC相似的水平时，我们发现鲍曼不动杆菌两个外排系统都泵出了多种化合物，但是在分离过程中，AdeABC的效率不如AcrAB-TolC。挤出亲脂性β-内酰胺，新生霉素和溴化乙锭，尽管它对四环素外排更有效。 AdeIJK在β-内酰胺，新生霉素和溴化乙锭的外排中比类似水平的AcrAB-TolC显着更有效，尽管在红霉素的外排中却不那么有效。因此，如果我们可以假设异源系统在大肠杆菌宿主中具有完整功能，那么这些结果将使我们能够定量地比较这些外排系统。

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