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Fluorescence Correlation Microscopy with Real-time Alignment Readout

机译:带有实时对准读数的荧光相关显微镜

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In confocal fluorescence correlation microscopy (FCM) it is important to ensure that the correlationmeasurement is actually performed at the chosen location of the three-dimensional image of the specimen. We present a confocal FCM design that provides an automatic real-time readout of the location in the confocal microscopic image, which is aligned with the detector of the fluorescence correlation spectrometer. The design accomplishes this without using any special positioning device. The design is based on an apertured fluorescence detector placed close to the back aperture of the objective lens and can be easily incorporated into virtually any confocal microscope. We demonstrate the method by performing FCM measurements of a dye diffusing on a cell membrane.
机译:在共聚焦荧光相关显微镜(FCM)中,重要的是要确保在标本的三维图像的选定位置实际执行相关测量。我们提出了一种共焦FCM设计,该设计可自动实时读取共焦显微图像中的位置,并与荧光相关光谱仪的检测器对齐。设计无需使用任何特殊的定位设备即可完成此操作。该设计基于靠近物镜后孔放置的带孔荧光检测器,几乎可以将其轻松集成到任何共聚焦显微镜中。我们通过对细胞膜上扩散的染料进行FCM测量来证明该方法。

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