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Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate

机译：多层基板观察到的激发光的荧光增强和反射

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摘要

Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al_(2)O_(3) was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al_(2)O_(3) had two peaks and one valley in the range from 0 to 300 nm of Al_(2)O_(3) thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al_(2)O_(3) thickness as well, and the maximum reflection was observed near the Al_(2)O_(3) thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the lambda/4 derived by a simple interference theory.
机译：使用荧光素，若丹明B，Cy3和Cy5作为荧光团，研究了用Ag和Al_（2）O_（3）制成的多层基板的荧光增强作用。 Al_（2）O_（3）的荧光增强变化在Al_（2）O_（3）厚度从0到300 nm的范围内有两个峰和一个谷，并且发现这些峰和谷周期性地出现。此外，研究了来自多层基板的激发光的反射。激发光的反射也根据Al_（2）O_（3）的厚度而周期性地变化，并且在峰值荧光增强的Al_（2）O_（3）的厚度附近观察到最大反射。已经发现，荧光增强的周期性变化和激发光的反射可以用简单干涉理论得出的λ/ 4的整数倍来解释。

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