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Thin-film optoacoustic transducers for subcellular Brillouin oscillation imaging of individual biological cells

机译：用于单个生物细胞亚细胞布里渊振荡成像的薄膜光声换能器

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摘要

At low frequencies ultrasound is a valuable tool to mechanically characterize and image biological tissues. There is much interest in using high-frequency ultrasound to investigate single cells. Mechanical characterization of vegetal and biological cells by measurement of Brillouin oscillations has been demonstrated using ultrasound in the GHz range. This paper presents a method to extend this technique from the previously reported single-point measurements and line scans into a high-resolution acoustic imaging tool. Our technique uses a three-layered metal-dielectric-metal film as a transducer to launch acoustic waves into the cell we want to study. The design of this transducer and measuring system is optimized to overcome the vulnerability of a cell to the exposure of laser light and heat without sacrificing the signal-to-noise ratio. The transducer substrate shields the cell from the laser radiation, efficiently generates acoustic waves, facilitates optical detection in transmission, and aids with heat dissipation away from the cell. This paper discusses the design of the transducers and instrumentation and presents Brillouin frequency images on phantom, fixed, and living cells. (C) 2015 Optical Society of America
机译：在低频下，超声是机械表征和成像生物组织的宝贵工具。使用高频超声研究单细胞引起了人们的极大兴趣。使用GHz范围内的超声波已证明通过测量布里渊振荡来对植物和生物细胞进行机械表征。本文提出了一种将这项技术从以前报道的单点测量和线扫描扩展到高分辨率声成像工具的方法。我们的技术使用三层金属介电金属膜作为换能器，将声波发射到我们要研究的单元中。该换能器和测量系统的设计经过优化，可以克服电池在暴露于激光和热中的脆弱性，而又不会牺牲信噪比。换能器基板可保护细胞免受激光辐射的影响，有效地产生声波，促进传输中的光学检测，并有助于将热量从细胞中散发出去。本文讨论了换能器和仪器的设计，并提出了幻影，固定和活细胞上的布里渊频率图像。 （C）2015年美国眼镜学会

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