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Features for instantaneous emissions of low-level infrared signals of glucokinase enzyme from pyrococcus furiosus

机译:激烈热球菌瞬时释放葡萄糖激酶低水平红外信号的功能

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摘要

A noncontact infrared (IR) imaging-based methodology and signal recovery tools are applied on an enzyme reaction as a test target. The method is implemented by a long-wave (8-12 mu m) IR microbolometer imaging array and a germanium-based IR optical vision. The reaction is carried out by the glucokinase, which produces a rapid exothermal release of energy that is weak, and, even worse, the IR video captured by the uncooled microbolometer detector is affected by spatial and temporal noise with specific complexities. Hitherto, IR-based signal recovery tools have worked with a standard acquisition frequency, which is clearly beyond the time scale of a real scenario. The implications of this (and similar) rapid reactions motivate the designs of a signal recovery method using prior information of the processes to extract and quantify the spontaneity of the enzymatic reaction in a three-dimensional (space and time) single and noncontact online measurement. (C) 2015 Optical Society of America
机译:基于非接触式红外(IR)成像的方法和信号恢复工具应用于酶反应作为测试目标。该方法通过长波(8-12微米)红外微辐射热成像仪成像阵列和基于锗的红外光学视觉实现。该反应由葡糖激酶进行,葡糖激酶会快速释放出微弱的能量,更糟糕的是,未冷却的微量辐射热检测器捕获的红外视频会受到具有特定复杂性的时空噪声的影响。迄今为止,基于IR的信号恢复工具已经以标准的采集频率工作,这显然超出了实际场景的时间范围。这种(和类似的)快速反应的含义激发了信号恢复方法的设计,该方法使用过程的先验信息来提取和量化三维(空间和时间)单次和非接触式在线测量中酶促反应的自发性。 (C)2015年美国眼镜学会

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