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Simple method to improve spatial resolution for in vivo two-photon fluorescence imaging

机译:改善体内双光子荧光成像空间分辨率的简单方法

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There is a growing effort to image single neurons in vivo, and observe their individual contribution to the brain's functional organization. This effort generally relies on two-photon imaging to explore the structure and activity of cortical columns extending beneath the brain's surface. The need to protect living tissue, however, demands the introduction of coverslips and similar objects that can modify the optics of the imaging beam. This paper develops three-dimensional (3D) analytical and numerical models to characterize and correct for the resulting degradation of image quality. We have illustrated the use of these models by describing a simple, practical technique to reduce the effect of spherical aberration for in vivo two-photon fluorescence experiments. (C) 2015 Optical Society of America
机译:人们正在努力在体内成像单个神经元,并观察它们对大脑功能组织的贡献。这项工作通常依靠双光子成像来探索在大脑表面下方延伸的皮质柱的结构和活动。然而,保护生物组织的需求要求引入盖玻片和类似的物体,它们可以改变成像光束的光学特性。本文开发了三维(3D)分析和数值模型,以表征和纠正由此导致的图像质量下降。我们通过描述一种简单的实用技术来减少体内两光子荧光实验中球差的影响,从而说明了这些模型的使用。 (C)2015年美国眼镜学会

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