• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >pH induced protein-scaffold biosynthesis of tunable shape gold nanoparticles
【24h】

pH induced protein-scaffold biosynthesis of tunable shape gold nanoparticles

机译：pH诱导的可调形状金纳米粒子的蛋白质支架生物合成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper, a pH-inductive protein-scaffold biosynthesis of shape-tunable crystalline gold nanoparticles at room temperature has been developed. By simple manipulation of the reaction solution's pH, anisotropic gold nanoparticles including spheres, triangles and cubes could be produced by incubating an aqueous solution of sodium tetrachloroaurate with Dolichomitriopsis diversiformis biomasses after immersion in ultrapure Millipore water overnight. A moss protein with molecular weight of about 71 kDa and pI of 4.9 was the primary biomolecule involved in the biosynthesis of gold nanoparticles. The secondary configuration of the proteins by CD spectrum implied that the moss protein could display different secondary configurations including random coil, α-helix and intermediate conformations between random coil and α-helix for the experimental pH solution. The growth process of gold nanoparticles further showed that the moss protein with different configurations provided the template scaffold for the shape-controlled biosynthesis of gold nanoparticles. The constrained shape of the gold nanoparticles, however, disappeared in boiled moss extract. The gold nanoparticles with designed morphology were successfully reconstructed using the moss protein purified from the gold nanoparticles. Structural characterizations by SEM, TEM and SAED showed that the triangular and cubic gold nanoparticles were single crystalline.
机译：在本文中，已经开发了在室温下形状可调的结晶金纳米粒子的pH诱导蛋白支架生物合成方法。通过简单地控制反应溶液的pH值，在超纯密理博尔水中浸泡过夜后，将四氯金酸钠水溶液与多形梭菌生物质一起孵育，即可制得包括球形，三角形和立方体的各向异性金纳米颗粒。分子量约为71 kDa且pI为4.9的苔藓蛋白是金纳米颗粒生物合成中涉及的主要生物分子。通过CD光谱对蛋白质进行的二级构型暗示，苔藓蛋白在实验pH溶液中可显示出不同的二级构型，包括无规卷曲，α-螺旋和无规卷曲与α-螺旋之间的中间构象。金纳米粒子的生长过程进一步表明，具有不同构型的苔藓蛋白为金纳米粒子的形状控制生物合成提供了模板支架。然而，金纳米颗粒的受约束形状在煮沸的苔藓提取物中消失了。使用从金纳米颗粒纯化的苔藓蛋白成功地重建了具有设计形态的金纳米颗粒。通过SEM，TEM和SAED进行的结构表征表明，三角形和立方金纳米颗粒是单晶的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号