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Facile one-pot preparation, surface functionalization, and toxicity assay of APTS-coated iron oxide nanoparticles

机译：APTS包覆的氧化铁纳米粒子的便捷一锅制备，表面功能化和毒性测定

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We report a facile approach to synthesizing 3-aminopropyltrimethoxysilane (APTS)-coated magnetic iron oxide (Fe _3O _4@APTS) nanoparticles (NPs) with tunable surface functional groups for potential biomedical applications. The Fe _3O _4 NPs with a mean diameter of 6.5nm were synthesized by a hydrothermal route in the presence of APTS. The formed amine-surfaced Fe _3O _4@APTS NPs were further chemically modified with acetic anhydride and succinic anhydride to generate neutral (Fe _3O _4@APTSAc) and negatively charged (Fe _3O _4@APTSSAH) NPs. These differently functionalized NPs were extensively characterized by x-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, thermogravimetry analysis, zeta potential measurements, and T 2 relaxometry. The cytotoxicity of the particles was evaluated by in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide colorimetric viability assay of cells along with microscopic observation of cell morphology. The hemocompatibility of the particles was assessed by in vitro hemolysis assay. We show that the hydrothermal approach enables an efficient modification of APTS onto the Fe _3O _4 NP surfaces and the formed NPs with different surface charge polarities are water-dispersible and colloidally stable. The acetylated Fe _3O _4@APTSAc NPs displayed good biocompatibility and hemocompatibility in the concentration range of 0100νgml ~(-1), while the pristine Fe _3O _4@APTS and Fe _3O _4@APTSSAH particles started to display slight cytotoxicity at a concentration of 10νgml ~(-1). The findings from this study suggest that the Fe _3O _4@APTS NPs synthesized by the one-pot hydrothermal route can be surface modified for various potential biomedical applications.
机译：我们报告一种简便的方法来合成3-氨基丙基三甲氧基硅烷（APTS）涂层的磁性氧化铁（Fe _3O _4 @ APTS）纳米粒子（NPs）具有可调的表面官能团，用于潜在的生物医学应用。在APTS存在下，通过水热法合成了平均直径为6.5nm的Fe _3O _4 NP。将形成的胺表面的Fe _3O _4 @ APTS NP进一步用乙酸酐和琥珀酸酐进行化学修饰，以生成中性（Fe _3O _4 @ APTSAc）和带负电荷的（Fe _3O _4 @ APTSSAH）NP。这些功能不同的NP通过X射线衍射，透射电子显微镜，傅立叶变换红外光谱，热重分析，ζ电位测量和T 2弛豫法进行了广泛表征。通过细胞的体外3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物比色生存力测定以及细胞形态的显微镜观察来评估颗粒的细胞毒性。通过体外溶血测定法评估颗粒的血液相容性。我们表明，水热方法能够将APTS有效地修饰到Fe _3O _4 NP表面上，并且所形成的具有不同表面电荷极性的NPs具有水分散性和胶体稳定性。乙酰化的Fe _3O _4 @ APTSAc NPs在0100νgml〜（-1）的浓度范围内表现出良好的生物相容性和血液相容性，而原始的Fe _3O _4 @ APTS和Fe _3O _4 @ APTSSAH颗粒在10νgml的浓度下开始表现出轻微的细胞毒性。 〜（-1）。这项研究的结果表明，通过一锅水热法合成的Fe _3O _4 @ APTS NPs可以进行表面改性，以用于各种潜在的生物医学应用。

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