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Probing cellular traction forces with magnetic nanowires and microfabricated force sensor arrays

机译：用磁性纳米线和微型力传感器阵列探测细胞的牵引力

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In this paper, the use of magnetic nanowires for the study of cellular response to force is demonstrated. High-aspect ratio Ni rods with diameter 300nm and lengths up to 20m were bound to or internalized by pulmonary artery smooth muscle cells (SMCs) cultured on arrays of flexible micropost force sensors. Forces and torques were applied to the cells by driving the nanowires with AC magnetic fields in the frequency range 0.110Hz, and the changes in cellular contractile forces were recorded with the microposts. These local stimulations yield global force reinforcement of the cells traction forces, but this contractile reinforcement can be effectively suppressed upon addition of a calcium channel blocker, ruthenium red, suggesting the role of calcium channels in the mechanical response. The responsiveness of the SMCs to actuation depends on the frequency of the applied stimulation. These results show that the combination of magnetic nanoparticles and micropatterned, flexible substrates can provide new approaches to the study of cellular mechanotransduction.
机译：在本文中，证明了使用磁性纳米线研究细胞对力的反应。直径为300nm且长度最大为20m的高纵横比镍棒与在柔性微柱力传感器阵列上培养的肺动脉平滑肌细胞（SMC）结合或内在化。通过用0.110Hz频率范围内的交流磁场驱动纳米线，将力和扭矩施加到细胞上，并用微柱记录细胞收缩力的变化。这些局部刺激产生细胞牵引力的整体力增强，但是在添加钙通道阻滞剂钌红后可以有效抑制这种收缩性增强，提示钙通道在机械反应中的作用。 SMC对致动的响应性取决于所施加刺激的频率。这些结果表明，磁性纳米粒子和微图案化的柔性基底的结合可以为细胞机械转导的研究提供新的方法。

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