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Towards understanding of poly-guanine activated fluorescent silver nanoclusters

机译：认识多鸟嘌呤激活的荧光银纳米簇

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摘要

It has been recently reported that the fluorescence of some DNA-templated silver nanoclusters (AgNCs) can be significantly enhanced upon by hybridizing with a partially complementary DNA containing a G-rich overhang near the AgNCs. This discovery has found a number of analytical applications but many fundamental questions remain to be answered. In this work, the photostability of these activated AgNCs is reported. After adding the G-rich DNA activator, the fluorescence intensity peaks in ~1 h and then starts to decay, where the decaying rate is much faster with light exposure. The lost fluorescence is recovered by adding NaBH_4, suggesting that the bleaching is an oxidative process. Once activated, the G-rich activator can be removed while the AgNCs still maintain most of their fluorescence intensity. UV-vis spectroscopy suggests that new AgNC species are generated upon hybridization with the activator. The base sequence and length of the template DNA have also been varied, leading to different emission colors and color change after hybridization. G-rich aptamers can also serve as activators. Our results indicate that activation of the fluorescence by G-rich DNA could be a convenient method for biosensor development since the unstable NaBH_4 is not required for the activation step.
机译：最近已经报道，通过与AgNC附近的含有富含G的突出端的部分互补DNA杂交，可以显着增强一些DNA模板化的银纳米簇（AgNC）的荧光。这一发现发现了许多分析应用，但仍有许多基本问题有待解答。在这项工作中，报告了这些激活的AgNC的光稳定性。加入富含G的DNA激活剂后，荧光强度会在约1小时内达到峰值，然后开始衰减，其中随着光线的照射，衰减速度要快得多。通过添加NaBH_4可以回收丢失的荧光，这表明漂白是一种氧化过程。激活后，可以去除富含G的激活剂，而AgNC仍保持其大部分荧光强度。紫外可见光谱表明，与激活剂杂交后会产生新的AgNC物种。模板DNA的碱基序列和长度也有所变化，导致杂交后产生不同的发射颜色和颜色变化。富含G的适体也可以用作激活剂。我们的结果表明，富含G的DNA激活荧光可能是生物传感器开发的便捷方法，因为激活步骤不需要不稳定的NaBH_4。

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