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首页> 外文期刊>Nanotechnology >Fabrication and characterization of gold nano-wires templated on virus-like arrays of tobacco mosaic virus coat proteins
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Fabrication and characterization of gold nano-wires templated on virus-like arrays of tobacco mosaic virus coat proteins

机译：在烟草花叶病毒外壳蛋白的病毒样阵列上模板化的金纳米线的制备和表征

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摘要

The rod-shaped plant virus tobacco mosaic virus (TMV) is widely used as a nano-fabrication template, and chimeric peptide expression on its major coat protein has extended its potential applications. Here we describe a simple bacterial expression system for production and rapid purification of recombinant chimeric TMV coat protein carrying C-terminal peptide tags. These proteins do not bind TMV RNA or form disks at pH 7. However, they retain the ability to self-assemble into virus-like arrays at acidic pH. C-terminal peptide tags in such arrays are exposed on the protein surface, allowing interaction with target species. We have utilized a C-terminal His-tag to create virus coat protein-templated nano-rods able to bind gold nanoparticles uniformly. These can be transformed into gold nano-wires by deposition of additional gold atoms from solution, followed by thermal annealing. The resistivity of a typical annealed wire created by this approach is significantly less than values reported for other nano-wires made using different bio-templates. This expression construct is therefore a useful additional tool for the creation of chimeric TMV-like nano-rods for bio-templating.
机译：杆状植物病毒烟草花叶病毒（TMV）被广泛用作纳米加工模板，嵌合肽在其主要外壳蛋白上的表达扩展了其潜在应用。在这里，我们描述了一个简单的细菌表达系统，用于生产和快速纯化带有C末端肽标签的重组嵌合TMV外壳蛋白。这些蛋白质在pH 7时不结合TMV RNA或形成圆盘。但是，它们保留了在酸性pH下自组装成病毒样阵列的能力。这种阵列中的C端肽标签暴露在蛋白质表面，从而可以与靶标物种相互作用。我们利用C端His-tag来创建病毒外壳蛋白模板化的纳米棒，该棒能够均匀结合金纳米颗粒。通过从溶液中沉积额外的金原子，然后进行热退火，可以将它们转变为金纳米线。通过这种方法创建的典型退火线的电阻率明显小于使用其他生物模板制作的其他纳米线的报告值。因此，该表达构建体是用于产生嵌合的TMV样纳米棒用于生物模板化的有用的附加工具。

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