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Controlling ferrofluid permeability across the blood–brain barrier model

机译：跨血脑屏障模型控制铁磁通透性

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In the present study, an in vitro blood–brain barrier model was developed using murine brain endothelioma cells (b.End3 cells). Confirmation of the blood–brain barrier model was completed by examining the permeability of FITC-Dextran at increasing exposure times up to 96 h in serum-free medium and comparing such values with values from the literature. After such confirmation, the permeability of five novel ferrofluid (FF) nanoparticle samples, GGB (ferrofluids synthesized using glycine, glutamic acid and BSA), GGC (glycine, glutamic acid and collagen), GGP (glycine, glutamic acid and PVA), BPC (BSA, PEG and collagen) and CPB (collagen, PVA and BSA), was determined using this blood–brain barrier model. All of the five FF samples were characterized by zeta potential to determine their charge as well as TEM and dynamic light scattering for determining their hydrodynamic diameter. Results showed that FF coated with collagen passed more easily through the blood–brain barrier than FF coated with glycine and glutamic acid based on an increase of 4.5% in permeability. Through such experiments, diverse magnetic nanomaterials (such as FF) were identified for: (1) MRI use since they were less permeable to penetrate the blood–brain barrier to avoid neural tissue toxicity (e.g. GGB) or (2) brain drug delivery since they were more permeable to the blood–brain barrier (e.g. CPB).
机译：在本研究中，使用鼠脑内皮细胞（b.End3细胞）建立了体外血脑屏障模型。通过检查FITC-右旋糖酐在无血清培养基中暴露时间延长至96小时后的渗透率，并将其与文献中的值进行比较，从而完成血脑屏障模型的确认。经过这样的确认，五个新的铁磁流体（FF）纳米颗粒样品，GGB（使用甘氨酸，谷氨酸和BSA合成的铁磁流体），GGC（甘氨酸，谷氨酸和胶原蛋白），GGP（甘氨酸，谷氨酸和PVA），BPC的渗透性（BSA，PEG和胶原蛋白）和CPB（胶原蛋白，PVA和BSA）是使用这种血脑屏障模型确定的。所有五个FF样品均通过zeta电位表征以确定其电荷，并通过TEM和动态光散射确定其流体动力学直径。结果表明，涂​​有胶原蛋白的FF比涂有甘氨酸和谷氨酸的FF更容易通过血脑屏障，这是因为渗透率提高了4.5％。通过这样的实验，鉴定出多种磁性纳米材料（例如FF）用于：（1）MRI的使用，因为它们不易渗透血脑屏障，从而避免了神经组织毒性（例如GGB）或（2）自从它们对血脑屏障（例如CPB）的渗透性更高。

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