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首页> 外文期刊>Nanotechnology >Ultrasonication-assisted one-step self-assembly preparation of biocompatible fluorescent-magnetic nanobeads for rare cancer cell detection
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Ultrasonication-assisted one-step self-assembly preparation of biocompatible fluorescent-magnetic nanobeads for rare cancer cell detection

机译：超声辅助一步法自组装生物相容性荧光磁性纳米珠用于罕见癌细胞检测

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摘要

Multifunctional nanomaterials simultaneously possessing attractive properties, such as strong fluorescent intensity, excellent superparamagnetic behavior, easy modification and good biocompatibility, are always desired in a wide range of applications. In this work, we present a facile ultrasonication-assisted one-step self-assembly strategy for the fabrication of smart fluorescent-magnetic nanobeads (FMNBs) without using a matrix. Via one-step ultrasonication, organic-soluble superparamagnetic nanoparticles (MNPs) and quantum dots (QDs) were automatically encapsulated by amphiphilic (2-hydroxyl-3-dodecanoxyl) propylcarboxymethylchitosans (HDP-CMCHSs) through hydrophobic interaction to form hydrophilic FMNBs, presenting a good QD fluorescent property and a strong MNP magnetic response. The outer surface of the FMNBs was derived from natural biopolymer chitosans, enabling FMNBs with good biocompatibility and convenience for biological modification. As prepared FMNBs can be easily modified with streptavidin, facilitating bioconjugation with biotin-labeled human epidermal growth factor (hEGF). hEGF-functionalized FMNBs are able to specifically recognize and capture rare target cells spiked in white blood cells, and the recovered cells can be further cultured for a long time. All of these excellent properties make nanobeads promising for circulating tumor cell (CTC) detection.
机译：同时具有广泛吸引力的多功能纳米材料总是需要吸引人的特性，例如强荧光强度，优异的超顺磁性行为，易改性和良好的生物相容性。在这项工作中，我们提出了一种无需使用基质即可制造智能荧光磁纳米珠（FMNB）的简便的超声辅助单步自组装策略。通过一步超声处理，两亲性（2-羟基-3-十二烷氧基）丙基羧甲基壳聚糖（HDP-CMCHS）通过疏水作用自动包裹有机可溶性超顺磁性纳米颗粒（MNP）和量子点（QD），形成亲水性FMNB。良好的QD荧光性能和强大的MNP磁响应。 FMNBs的外表面衍生自天然生物聚合物壳聚糖，使FMNBs具有良好的生物相容性和生物修饰的便利性。所制备的FMNBs可以很容易地用链霉亲和素修饰，从而促进与生物素标记的人表皮生长因子（hEGF）的生物结合。 hEGF功能化的FMNBs能够特异性识别和捕获掺入白细胞的稀有靶细胞，并且回收的细胞可以长期培养。所有这些优异的性能使纳米珠有望用于循环肿瘤细胞（CTC）检测。

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