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Silver nanoparticle enhanced Raman scattering-based lateral flow immunoassays for ultra-sensitive detection of the heavy metal chromium

机译：银纳米颗粒增强拉曼散射为基础的侧向免疫测定法，用于重金属铬的超灵敏检测

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摘要

We report a simple and ultra-sensitive surface enhanced Raman scattering (SERS) strip sensor based on silver nanoparticles (AgNPs) and lateral flow immunoassays (LFIAs). LFIAs are inexpensive, simple, portable and robust, thus making them commonplace in medicine, agriculture and food safety. However, their applications are limited due to the low signal intensity of the color-formation reaction based on the label accumulation. SERS is a powerful molecular spectroscopy technique for ultra-detection, which is based on the enhancement of the inelastic scattering from molecules located near nanostructured metallic surfaces when the molecules are illuminated and the surface plasmons are excited. Because of the rapidity and robustness of LFIAs and the high sensitivity of SERS, we introduce SERS into LFIAs (SERS-LFIA). Our SERS-LFIA demonstrates fast, excellent performance and is suitable for the semiquantitative examination of ultratrace analytes (Cr3+), with the limit of the detection (LOD) as low as 10(-5) ng mL(-1), which is 10(5)-fold more highly sensitive than those previously used to detect Cr3+ within 15 min.
机译：我们报告了基于银纳米颗粒（AgNPs）和侧向流免疫测定（LFIAs）的简单且超灵敏的表面增强拉曼散射（SERS）条形传感器。 LFIA价格便宜，简单，便携式且坚固耐用，因此使其在医学，农业和食品安全中司空见惯。然而，由于基于标签积累的颜色形成反应的低信号强度，它们的应用受到限制。 SERS是一种用于超检测的强大分子光谱技术，该技术基于当分子被照射并激发表面等离激元时，来自纳米结构金属表面附近分子的非弹性散射的增强。由于LFIA的快速性和鲁棒性以及SERS的高灵敏度，我们将SERS引入LFIA（SERS-LFIA）。我们的SERS-LFIA表现出快速，出色的性能，适用于超痕量分析物（Cr3 +）的半定量检测，检测限（LOD）低至10（-5）ng mL（-1），即10 （15）比以前在15分钟内检测Cr3 +的灵敏度高三倍。

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