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首页> 外文期刊>Analytica chimica acta >Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips
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Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips

机译：使用表面增强拉曼散射侧流动免疫条带的超声检测禽流感A（H7N9）病毒

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摘要

The development of biosensors that are portable, low-cost, and quantitative has long been sought for rapid, on-site, and timely detection of avian influenza virus (AIV). In this study, an antibody-based Raman lateral flow immunoassay strip was developed to detect AIV H7N9. This LFIA strip used a novel core-shell structure material, AuAg4-ATP@AgNPs, as a Raman probe. An antibody specific for AIV and goat anti-mouse IgG antibody were immobilized on a nitrocellulose membrane as the test and control lines, respectively. Accumulation of antibody-virus-antibody-Raman probe complex at the test line could be visualized by the naked eye, and the Raman signal could be quantified using a portable Raman instrument. The testing process for the SERS-based LFIA strips could be completed in 20 min, which avoided the time-cost of current methods for AIV analysis. In our SERS-based biosensor, we estimated the limit of detection (LOD) for H7N9 to be 0.0018 HAU. This value is approximately three orders of magnitude more sensitive than the corresponding HA assays. When testing real sample, the results of the strip test were in accordance with those from real-time PCR testing. In conclusion, the SERS-based LFIA strip proposed in this study shows tremendous potential to detect targets quickly and sensitively using an elegantly simple method. (C) 2018 Elsevier B.V. All rights reserved.
机译：长期以来，长期寻求禽流感病毒（AIV）的快速，现场和及时检测长期以来的生物传感器的发展。在该研究中，开发了一种基于抗体的拉曼横向流动免疫测定条以检测AIV H7N9。该LFIA条带有一种新型芯壳结构材料，AuAG4-ATP @ AGNPS作为拉曼探头。将针对AIV和山羊抗小鼠IgG抗体特异的抗体固定在硝酸纤维素膜上作为试验和对照系。在测试线处的抗体 - 病毒 - 抗体 - 拉曼探针复合物可以通过肉眼可视化，并且可以使用便携式拉曼仪器量化拉曼信号。基于SERS的LFIA条带的测试过程可以在20分钟内完成，这避免了当前AIV分析方法的时间成本。在我们基于SERS的生物传感器中，我们估计了H7N9的检测（LOD）的极限为0.0018 HAU。该值大约比相应的HA测定更敏感的三个数量级。在测试真实样本时，条带测试的结果符合实时PCR测试的结果。总之，本研究提出的基于SERS的LFIA条带显示了使用优雅简单的方法快速和灵敏地检测目标的巨大潜力。 （c）2018 Elsevier B.v.保留所有权利。

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  • 来源
    《Analytica chimica acta》 |2019年第2019期|共9页
  • 作者

    Xiao Meng; Xie Kaixin; Dong Xiaohui; Wang Lei; Huang Caihong; Xu Fei; Xiao Wei; Jin Meilin; Huang Boyan; Tang Yong;

  • 作者单位

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Huazhong Agr Univ Coll Vet Med State Key Lab Agr Microbiol Wuhan 430070 Hubei Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Huazhong Agr Univ Coll Vet Med State Key Lab Agr Microbiol Wuhan 430070 Hubei Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

    Jinan Univ Guangdong Prov Engn Res Ctr Antibody Drug &

    Immun Dept Bioengn Guangzhou 510632 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
  • 关键词

    Avian influenza virus; AuAg4-ATP@AgNPs; Surface-enhanced Raman scattering; Lateral flow immunoassay strips;

    机译：禽流感病毒;Auag4-ATP @ AGNPS;表面增强拉曼散射;侧面流动免疫测定条;

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