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Analytical characterization using surface-enhanced Raman scattering (SERS) and microfluidic sampling

机译：使用表面增强拉曼散射（SERS）和微流体采样进行分析表征

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摘要

With the rapid development of analytical techniques, it has become much easier to detect chemical and biological analytes, even at very low detection limits. In recent years, techniques based on vibrational spectroscopy, such as surface enhanced Raman spectroscopy (SERS), have been developed for non-destructive detection of pathogenic microorganisms. SERS is a highly sensitive analytical tool that can be used to characterize chemical and biological analytes interacting with SERS-active substrates. However, it has always been a challenge to obtain consistent and reproducible SERS spectroscopic results at complicated experimental conditions. Microfluidics, a tool for highly precise manipulation of small volume liquid samples, can be used to overcome the major drawbacks of SERS-based techniques. High reproducibility of SERS measurement could be obtained in continuous flow generated inside microfluidic devices. This article provides a thorough review of the principles, concepts and methods of SERS-microfluidic platforms, and the applications of such platforms in trace analysis of chemical and biological analytes.
机译：随着分析技术的飞速发展，即使在非常低的检测限下，化学和生物分析物的检测也变得更加容易。近年来，已经开发出基于振动光谱的技术，例如表面增强拉曼光谱（SERS），用于病原微生物的非破坏性检测。 SERS是一种高度灵敏的分析工具，可用于表征与SERS活性底物相互作用的化学和生物分析物。然而，在复杂的实验条件下获得一致且可重复的SERS光谱结果一直是一个挑战。微流体，一种用于小体积液体样品的高精度处理的工具，可用于克服基于SERS的技术的主要缺点。在微流体装置内部产生的连续流中，可以获得SERS测量的高重复性。本文全面回顾了SERS微流体平台的原理，概念和方法，以及此类平台在化学和生物分析物的痕量分析中的应用。

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