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Detection of DNA and poly-l-lysine using CVD graphene-channel FET biosensors

机译：使用CVD石墨烯通道FET生物传感器检测DNA和聚l-赖氨酸

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A graphene channel field-effect biosensor is demonstrated for detecting the binding of double-stranded DNA and poly-l-lysine. Sensors consist of chemical vapor deposition graphene transferred using a clean, etchant-free transfer method. The presence of DNA and poly-l-lysine are detected by the conductance change of the graphene transistor. A readily measured shift in the Dirac voltage (the voltage at which the graphene's resistance peaks) is observed after the graphene channel is exposed to solutions containing DNA or polyl-l-ysine. The 'Dirac voltage shift' is attributed to the binding/unbinding of charged molecules on the graphene surface. The polarity of the response changes to positive direction with poly-l-lysine and negative direction with DNA. This response results in detection limits of 8 pM for 48.5 kbp DNA and 11 pM for poly-l-lysine. The biosensors are easy to fabricate, reusable and are promising as sensors of a wide variety of charged biomolecules.
机译：石墨烯通道场效应生物传感器被证明用于检测双链DNA和聚l赖氨酸的结合。传感器由使用清洁的无蚀刻剂转移方法转移的化学气相沉积石墨烯组成。通过石墨烯晶体管的电导变化检测DNA和聚-1-赖氨酸的存在。在石墨烯通道暴露于含有DNA或聚1-1赖氨酸的溶液后，观察到狄拉克电压（石墨烯的电阻峰值处的电压）容易测量到的变化。 “狄拉克电压偏移”归因于石墨烯表面上带电分子的结合/解结合。响应的极性在多聚赖氨酸的情况下变为正方向，而在DNA情况下变为负方向。该响应导致48.5 kbp DNA的检测限为8 pM，而聚-1-赖氨酸的检测限为11 pM。该生物传感器易于制造，可重复使用，并有望用作各种带电生物分子的传感器。

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