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Biocompatible capped iron oxide nanoparticles for Vibrio cholerae detection

机译：生物相容性加帽氧化铁纳米颗粒用于霍乱弧菌检测

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We report the studies relating to fabrication of an efficient immunosensor for Vibrio cholerae detection. Magnetite (iron oxide (Fe3O4)) nanoparticles (NPs) have been synthesized by the co-precipitation method and capped by citric acid (CA). These NPs were electrophoretically deposited onto indium-tin-oxide (ITO)-coated glass substrate and used for immobilization of monoclonal antibodies against Vibrio cholerae (Ab) and bovine serum albumin (BSA) for Vibrio cholerae detection using an electrochemical technique. The structural and morphological studies of Fe3O4 and CA-Fe3O4/ITO were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) techniques. The average crystalline size of Fe3O4, CA-Fe3O4 nanoparticles obtained were about 29 +/- 1 nm and 37 +/- 1 nm, respectively. The hydrodynamic radius of the nanoparticles was found to be 77.35 nm (Fe3O4) and 189.51 nm (CA-Fe3O4) by DLS measurement. The results of electrochemical response studies of the fabricated BSA/Ab/CAFe(3)O(4)/ITO immunosensor exhibits a good detection range of 12.5-500 ng mL(-1) with a low detection limit of 0.32 ng mL(-1), sensitivity 0.03 Omegag ml(-1) cm(-2), and reproducibility more than 11 times.
机译：我们报告有关霍乱弧菌检测有效的免疫传感器制造研究。通过共沉淀法合成了磁铁矿（氧化铁（Fe3O4））纳米颗粒（NPs），并用柠檬酸（CA）封端。将这些NP电泳沉积在涂有氧化铟锡（ITO）的玻璃基板上，并用于固定抗霍乱弧菌（Ab）和牛血清白蛋白（BSA）的单克隆抗体，以使用电化学技术检测霍乱弧菌。 Fe3O4和CA-Fe3O4 / ITO的结构和形态研究通过X射线衍射（XRD），透射电子显微镜（TEM），傅立叶变换红外（FTIR）光谱和动态光散射（DLS）技术进行了表征。获得的Fe 3 O 4，CA-Fe 3 O 4纳米颗粒的平均晶体尺寸分别为约29 +/- 1nm和37 +/- 1nm。通过DLS测量发现纳米颗粒的流体力学半径为77.35nm（Fe 3 O 4）和189.51nm（CA-Fe 3 O 4）。制成的BSA / Ab / CAFe（3）O（4）/ ITO免疫传感器的电化学响应研究结果显示，良好的检测范围为12.5-500 ng mL（-1），低检测限为0.32 ng mL（- 1），灵敏度0.03Ω/ ng ml（-1）cm（-2）和可重复性超过11倍。

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