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Relevance of fiber integrated gelatin-nanohydroxyapatite composite scaffold for bone tissue regeneration

机译：纤维集成明胶-纳米羟基磷灰石复合支架与骨组织再生的相关性

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摘要

Porous nanohydroxyapatite (nanoHA) is a promising bone substitute, but it is brittle, which limits its utility for load bearing applications. To address this issue, herein, biodegradable electrospun microfibrous sheets of poly(L-lactic acid)-(PLLA)-polyvinyl alcohol (PVA) were incorporated into a gelatin-nanoHA matrix which was investigated for its mechanical properties, the physical integration of the fibers with the matrix, cell infiltration, osteogenic differentiation and bone regeneration. The inclusion of sacrificial fibers like PVA along with PLLA and leaching resulted in improved cellular infiltration towards the center of the scaffold. Furthermore, the treatment of PLLA fibers with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide enhanced their hydrophilicity, ensuring firm anchorage between the fibers and the gelatin-HA matrix. The incorporation of PLLA microfibers within the gelatin-nanoHA matrix reduced the brittleness of the scaffolds, the effect being proportional to the number of layers of fibrous sheets in the matrix. The proliferation and osteogenic differentiation of human adipose-derived mesenchymal stem cells was augmented on the fibrous scaffolds in comparison to those scaffolds devoid of fibers. Finally, the scaffold could promote cell infiltration, together with bone regeneration, upon implantation in a rabbit femoral cortical defect within 4 weeks. The bone regeneration potential was significantly higher when compared to commercially available HA (Surgiwear T). Thus, this biomimetic, porous, 3D composite scaffold could be offered as a promising candidate for bone regeneration in orthopedics.
机译：多孔纳米羟基磷灰石（nanoHA）是一种有前途的骨替代品，但它很脆，限制了其在承重应用中的实用性。为了解决这个问题，在本文中，将可生物降解的聚（L-乳酸）-（PLLA）-聚乙烯醇（PVA）的电纺微纤维片材掺入明胶-nanoHA基质中，研究其机械性能，纤维的物理整合性。纤维与基质，细胞浸润，成骨分化和骨再生。牺牲纤维（如PVA）与PLLA以及浸出的结合导致细胞向支架中心的浸润得到改善。此外，用1-乙基-3-（3-二甲基氨基丙基）碳二亚胺处理PLLA纤维增强了其亲水性，确保了纤维与明胶HA基质之间的牢固锚固。在明胶-nanoHA基质中掺入PLLA超细纤维可降低支架的脆性，其效果与基质中纤维片的层数成正比。与不含纤维的支架相比，人源性间充质干细胞在纤维支架上的增殖和成骨分化得到增强。最后，在植入兔股骨皮质缺损后4周内，该支架可以促进细胞浸润以及骨骼再生。与市售的HA（Surgiwear T）相比，其骨再生潜力明显更高。因此，这种仿生的，多孔的3D复合支架可以作为骨科骨再生的有前途的候选者。

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