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Dielectrophoretic stretching of DNA tethered to a fiber tip

机译：束缚在纤维末端的DNA的介电泳拉伸

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In this work, we studied the stretching of. phage DNA molecules immobilized on an optical fiber tip attached to a force sensitive tuning fork under ac electric fields. We designed a two electrodes stretching system in a small chamber: one is a gold-coated optical fiber tip electrode, and the other is a gold-coated flat electrode. By applying a dielectrophoretic (DEP) force, the immobilized. DNA molecules on the tip are stretched and the stretching process is monitored by a fluorescent microscope. The DNA stretching in three-dimensional space is optimized by varying electrode shape, electrode gap distance, ac frequency, and solution conductivity. By observing the vibrational amplitude change of a quartz tuning fork, we measured the effects due to Joule heating and the DEP force on the tethered DNA molecules in solution. This work demonstrates a method to manipulate and characterize immobilized. DNA molecules on a probe tip for further study of single DNA molecules.
机译：在这项工作中，我们研究了拉伸。噬菌体DNA分子固定在光纤尖端上，该尖端与交流电场作用下的力敏感音叉相连。我们在一个小腔室内设计了两个电极拉伸系统：一个是镀金的光纤尖端电极，另一个是镀金的扁平电极。通过施加介电泳（DEP）力，将其固定。尖端上的DNA分子被拉伸，并通过荧光显微镜监控拉伸过程。通过改变电极形状，电极间隙距离，交流频率和溶液电导率，可以优化在三维空间中拉伸的DNA。通过观察石英音叉的振动幅度变化，我们测量了焦耳热和DEP力对溶液中束缚的DNA分子的影响。这项工作演示了一种操作和表征固定化的方法。探针尖端上的DNA分子，用于进一步研究单个DNA分子。

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