• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >Xanthan gum stabilized PEGylated gold nanoparticles for improved delivery of curcumin in cancer
【24h】

Xanthan gum stabilized PEGylated gold nanoparticles for improved delivery of curcumin in cancer

机译：黄原胶稳定的聚乙二醇化金纳米颗粒可改善姜黄素在癌症中的递送

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In recent years, gold nanoparticles (AuNPs) have received immense interest in various biomedical applications including drug delivery, photothermal ablation of cancer and imaging agent for cancer diagnosis. However, the synthesis of AuNPs poses challenges due to the poor reproducibility and stability of the colloidal system. In the present work, we developed a one step, facile procedure for the synthesis of AuNPs from hydrogen tetrachloroaurate (III) hydrate (HAuCl4. 3H(2)O) by using ascorbic acid and xanthan gum (XG) as reducing agent and stabilizer, respectively. The effect of concentrations of HAuCl4, 3H(2)O, ascorbic acid and methoxy polyethylene glycol-thiol (mPEG800-SH) were optimized and it was observed that stable AuNPs were formed at concentrations of 0.25 mM, 50 mu M and 1 mM for HAuCl4.3H2O, ascorbic acid, and mPEG800-SH, respectively. The XG stabilized, deep red wine colored AuNPs (XG-AuNPs) were obtained by drop-wise addition of aqueous solution of ascorbic acid (50 mM) and XG (1.5 mg ml(-1)). Synthesized XG-AuNPs showed lambda max at 540 nm and a mean hydrodynamic diameter of 80 +/- 3 nm. PEGylation was performed with mPEG800-SH to obtain PEGylated XG-AuNPs (PX-AuNPs) and confirmed by Ellman's assay. No significant shift observed in lambda max and hydrodynamic diameter between XG-AuNPs and PX-AuNPs. Colloidal stability of PX-AuNPs was studied in normal saline, buffers within a pH range of 1.2-7.4, DMEM complete medium and in normal storage condition at 4 degrees C. Further, water soluble curcumin was prepared using PVP-K30 as solid dispersion and loaded on to PX-AuNPs (CPX-AuNPs), and evaluated for cellular uptake and cytotoxicity in Murine melanoma (B16F10) cells. Time and concentration dependent studies using CPX-AuNPs showed efficient uptake and decreased cell viability compared to free curcumin.
机译：近年来，金纳米颗粒（AuNPs）在各种生物医学应用（包括药物输送，癌症的光热消融和用于癌症诊断的成像剂）中引起了极大的兴趣。然而，由于胶体系统的低再现性和稳定性，AuNP的合成提出了挑战。在目前的工作中，我们开发了一种简单的步骤，以抗坏血酸和黄原胶（XG）为还原剂和稳定剂，从四氯金酸氢盐（III）水合物（HAuCl4。3H（2）O）合成AuNP，分别。优化了HAuCl4、3H（2）O，抗坏血酸和甲氧基聚乙二醇硫醇（mPEG800-SH）浓度的影响，观察到在0.25 mM，50μM和1 mM浓度下会形成稳定的AuNP。 HAuCl4.3H2O，抗坏血酸和mPEG800-SH。通过逐滴添加抗坏血酸水溶液（50 mM）和XG（1.5 mg ml（-1）），可以得到XG稳定的深酒红色AuNP（XG-AuNPs）。合成的XG-AuNPs在540 nm处显示最大λ，平均流体动力学直径为80 +/- 3 nm。用mPEG800-SH进行PEG化，以获得PEG化的XG-AuNPs（PX-AuNPs），并通过Ellman法进行了确认。没有观察到XG-AuNPs和PX-AuNPs之间的最大λ和流体动力学直径发生明显变化。在生理盐水，pH范围为1.2-7.4的缓冲液，DMEM完全培养基和4°C的正常储存条件下研究了PX-AuNPs的胶体稳定性。此外，使用PVP-K30作为固体分散体制备了水溶性姜黄素，加载到PX-AuNPs（CPX-AuNPs）上，并评估小鼠黑素瘤（B16F10）细胞的细胞摄取和细胞毒性。与游离姜黄素相比，使用CPX-AuNPs进行的时间和浓度依赖性研究显示有效摄取并降低了细胞活力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号