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首页> 外文期刊>Nucleic Acids Research >A biochemically active MCM-like helicase in Bacillus cereus.
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A biochemically active MCM-like helicase in Bacillus cereus.

机译：蜡样芽孢杆菌中具有生化活性的MCM样解旋酶。

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摘要

The mini-chromosome maintenance (MCM) proteins serve as the replicative helicases in archaea and eukaryotes. Interestingly, an MCM homolog was identified, by BLAST analysis, within a phage integrated in the bacterium Bacillus cereus (Bc). BcMCM is only related to the AAA region of MCM-helicases; the typical amino-terminus is missing and is replaced by a segment with weak homology to primases. We show that BcMCM displays 3'[rightward arrow]5' helicase and ssDNA-stimulated ATPase activity, properties that arise from its conserved AAA domain. Isolated BcMCM is a monomer in solution but likely forms the functional oligomer in vivo. We found that the BcMCM amino-terminus can bind ssDNA and harbors a zinc atom, both hallmarks of the typical MCM amino-terminus. No BcMCM-catalyzed primase activity could be detected. We propose that the divergent amino-terminus of BcMCM is a paralog of the corresponding region of MCM-helicases. A divergent amino terminus makes BcMCM a useful model for typical MCM-helicases since it accomplishes the same function using an apparently unrelated structure.
机译：微型染色体维持（MCM）蛋白可作为古细菌和真核生物中的复制解旋酶。有趣的是，通过BLAST分析，在蜡样芽胞杆菌（Bcillus cereus，Bc）中整合的噬菌体中鉴定出MCM同源物。 BcMCM仅与MCM螺旋抗体的AAA区有关;典型的氨基末端缺失，并被与primases同源性较弱的片段所取代。我们显示BcMCM显示3'[向右箭头] 5'解旋酶和ssDNA刺激的ATPase活性，其保守的AAA结构域产生的特性。分离的BcMCM是溶液中的单体，但可能在体内形成功能性低聚物。我们发现BcMCM氨基末端可以结合ssDNA并带有一个锌原子，这是典型MCM氨基末端的两个标志。没有检测到BcMCM催化的启动酶活性。我们提出，BcMCM的不同氨基末端是MCM-螺旋酶相应区域的旁系同源物。分歧的氨基末端使BcMCM成为典型MCM螺旋分子的有用模型，因为它使用明显无关的结构完成相同的功能。

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