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首页> 外文期刊>Nucleic Acids Research >Human PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities.
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Human PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities.

机译：人PSF与RAD51结合并调节其同源配对和链交换活性。

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摘要

RAD51, a eukaryotic recombinase, catalyzes homologous-pairing and strand-exchange reactions, which are essential steps in homologous recombination and recombinational repair of double strand breaks. On the other hand, human PSF was originally identified as a component of spliceosomes, and its multiple functions in RNA processing, transcription and DNA recombination were subsequently revealed. In the present study, we found that PSF directly interacted with RAD51. PSF significantly enhanced RAD51-mediated homologous pairing and strand exchange at low RAD51 concentrations; however, in contrast, it inhibited these RAD51-mediated recombination reactions at the optimal RAD51 concentration. Deletion analyses revealed that the N-terminal region of PSF possessed the RAD51- and DNA-binding activities, but the central region containing the RNA-recognition motifs bound neither RAD51 nor DNA. These results suggest that PSF may have dual functions in homologous recombination and RNA processing through its N-terminal and central regions, respectively.
机译：RAD51是一种真核重组酶，可催化同源配对和链交换反应，这是同源重组和双链断裂的重组修复中必不可少的步骤。另一方面，人类PSF最初被鉴定为剪接体的组成部分，随后揭示了其在RNA加工，转录和DNA重组中的多种功能。在本研究中，我们发现PSF与RAD51直接相互作用。 PSF在低RAD51浓度下显着增强RAD51介导的同源配对和链交换。然而，相反，它在最佳RAD51浓度下抑制了这些RAD51介导的重组反应。缺失分析表明，PSF的N端区域具有RAD51和DNA的结合活性，但包含RNA识别基序的中央区域既不结合RAD51也未结合DNA。这些结果表明PSF可能分别通过其N-末端和中央区域在同源重组和RNA加工中具有双重功能。

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