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首页> 外文期刊>Nucleic Acids Research >Diverse transcription influences can be insulated by the Drosophila SF1 chromatin boundary.
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Diverse transcription influences can be insulated by the Drosophila SF1 chromatin boundary.

机译：果蝇SF1染色质边界可以隔离各种转录影响。

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摘要

Chromatin boundaries regulate gene expression by modulating enhancer-promoter interactions and insulating transcriptional influences from organized chromatin. However, mechanistic distinctions between these two aspects of boundary function are not well understood. Here we show that SF1, a chromatin boundary located in the Drosophila Antennapedia complex (ANT-C), can insulate the transgenic miniwhite reporter from both enhancing and silencing effects of surrounding genome, a phenomenon known as chromosomal position effect or CPE. We found that the CPE-blocking activity associates with different SF1 sub-regions from a previously characterized insulator that blocks enhancers in transgenic embryos, and is independent of GAF-binding sites essential for the embryonic insulator activity. We further provide evidence that the CPE-blocking activity cannot be attributed to an enhancer-blocking activity in the developing eye. Our results suggest that SF1 contains multiple non-overlapping activities that block diverse transcriptional influences from embryonic or adult enhancers, and from positive and negative chromatin structure. Such diverse insulating capabilities are consistent with the proposed roles of SF1 to functionally separate fushi tarazu (ftz), a non-Hox gene, from the enhancers and the organized chromatin of the neighboring Hox genes.
机译：染色质边界通过调节增强子-启动子相互作用和隔离有组织染色质的转录影响来调节基因表达。但是，边界函数的这两个方面之间的机械区别还不太清楚。在这里，我们显示SF1，位于果蝇触角复合体（ANT-C）中的染色质边界，可以隔离转基因微型白报告基因免受周围基因组的增强和沉默作用，这种现象称为染色体位置效应或CPE。我们发现，CPE阻断活性与先前表征的绝缘子具有不同的SF1子区域，该绝缘子阻断了转基因胚胎中的增强子，并且独立于胚胎绝缘子活性所必需的GAF结合位点。我们进一步提供证据，表明CPE阻断活性不能归因于发育中的眼睛的增强剂阻断活性。我们的结果表明，SF1包含多种非重叠活性，可阻止来自胚胎或成年增强子以及阳性和阴性染色质结构的多种转录影响。这种多样的绝缘能力与SF1在功能上将非Hox基因fushi tarazu（ftz）与邻近的Hox基因的增强子和有组织的染色质分离开来的作用相一致。

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