...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic Acids Research >Insights into the molecular determinants involved in cap recognition by the vaccinia virus D10 decapping enzyme
【24h】

Insights into the molecular determinants involved in cap recognition by the vaccinia virus D10 decapping enzyme

机译：深入了解牛痘病毒D10解盖酶参与帽识别的分子决定因素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Decapping enzymes are required for the removal of the 5'-end (m7)GpppN cap of mRNAs to allow their decay in cells. While many cap-binding proteins recognize the cap structure via the stacking of the methylated guanosine ring between two aromatic residues, the precise mechanism of cap recognition by decapping enzymes has yet to be determined. In order to get insights into the interaction of decapping enzymes with the cap structure, we studied the vaccinia virus D10 decapping enzyme as a model to investigate the important features for substrate recognition by the enzyme. We demonstrate that a number of chemically modified purines can competitively inhibit the decapping reaction, highlighting the molecular features of the cap structure that are required for recognition by the enzyme, such as the nature of the moiety at positions 2 and 6 of the guanine base. A 3D structural model of the D10 protein was generated which suggests amino acids implicated in cap binding. Consequently, we expressed 17 mutant proteins with amino acid substitutions in the active site of D10 and found that eight are critical for the decapping activity. These data underscore the functional features involved in the non-canonical cap-recognition by the vaccinia virus D10 decapping enzyme.
机译：需要去壳酶才能去除mRNA的5'-末端（m7）GpppN帽，以使其在细胞中腐烂。尽管许多帽结合蛋白通过两个芳族残基之间甲基化鸟嘌呤环的堆积来识别帽结构，但是通过去盖酶的帽识别的精确机理尚未确定。为了深入了解脱盖酶与帽结构的相互作用，我们研究了牛痘病毒D10脱盖酶作为模型，以研究该酶对底物识别的重要特征。我们证明了许多化学修饰的嘌呤可以竞争性地抑制脱盖反应，突出了被酶识别所需的帽结构的分子特征，例如鸟嘌呤碱基的位置2和6的部分的性质。产生了D10蛋白的3D结构模型，其暗示了与帽结合有关的氨基酸。因此，我们在D10的活性位点表达了17个具有氨基酸取代的突变蛋白，并且发现有8个对于去盖活性至关重要。这些数据强调了牛痘病毒D10脱盖酶参与非经典帽识别的功能特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号