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首页> 外文期刊>Nucleic Acids Research >Conservation of mRNA secondary structures may filter out mutations in Escherichia coli evolution
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Conservation of mRNA secondary structures may filter out mutations in Escherichia coli evolution

机译：mRNA二级结构的保守性可能会滤除大肠杆菌进化过程中的突变

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Recent reports indicate that mutations in viral genomes tend to preserve RNA secondary structure, and those mutations that disrupt secondary structural elements may reduce gene expression levels, thereby serving as a functional knockout. In this article, we explore the conservation of secondary structures of mRNA coding regions, a previously unknown factor in bacterial evolution, by comparing the structural consequences of mutations in essential and nonessential Escherichia coli genes accumulated over 40 000 generations in the course of the 'long-term evolution experiment'. We monitored the extent to which mutations influence minimum free energy (MFE) values, assuming that a substantial change in MFE is indicative of structural perturbation. Our principal finding is that purifying selection tends to eliminate those mutations in essential genes that lead to greater changes of MFE values and, therefore, may be more disruptive for the corresponding mRNA secondary structures. This effect implies that synonymous mutations disrupting mRNA secondary structures may directly affect the fitness of the organism. These results demonstrate that the need to maintain intact mRNA structures imposes additional evolutionary constraints on bacterial genomes, which go beyond preservation of structure and function of the encoded proteins.
机译：最近的报道表明，病毒基因组中的突变倾向于保留RNA二级结构，而那些破坏二级结构元件的突变可能会降低基因表达水平，从而起到功能性敲除的作用。在本文中，我们通过比较“长”过程中累积了4万多代的必需和非必需大肠杆菌基因突变的结构后果，探索了细菌编码中先前未知的mRNA编码区二级结构的保守性。学期进化实验”。假设MFE的实质性变化指示结构扰动，我们监测了突变影响最小自由能（MFE）值的程度。我们的主要发现是，纯化选择趋向于消除必需基因中的那些突变，这些突变会导致MFE值发生更大的变化，因此可能对相应的mRNA二级结构更具破坏性。这种作用暗示破坏mRNA二级结构的同义突变可能直接影响生物体的适应性。这些结果表明，维持完整的mRNA结构的需要在细菌基因组上施加了额外的进化限制，这超出了编码蛋白的结构和功能的保留范围。

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