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首页> 外文期刊>Nucleic Acids Research >Identification of discrete classes of small nucleolar RNA featuring different ends and RNA binding protein dependency
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Identification of discrete classes of small nucleolar RNA featuring different ends and RNA binding protein dependency

机译：鉴定具有不同末端和RNA结合蛋白依赖性的离散小核仁RNA类型

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摘要

Small nucleolar RNAs (snoRNAs) are among the first discovered and most extensively studied group of small non-coding RNA. However, most studies focused on a small subset of snoRNAs that guide the modification of ribosomal RNA. In this study, we annotated the expression pattern of all box C/D snoRNAs in normal and cancer cell lines independent of their functions. The results indicate that C/D snoRNAs are expressed as two distinct forms differing in their ends with respect to boxes C and D and in their terminal stem length. Both forms are overexpressed in cancer cell lines but display a conserved end distribution. Surprisingly, the long forms are more dependent than the short forms on the expression of the core snoRNP protein NOP58, thought to be essential for C/D snoRNA production. In contrast, a subset of short forms are dependent on the splicing factor RB-FOX2. Analysis of the potential secondary structure of both forms indicates that the k-turn motif required for binding of NOP58 is less stable in short forms which are thus less likely to mature into a canonical snoRNP. Taken together the data suggest that C/D snoRNAs are divided into at least two groups with distinct maturation and functional preferences.
机译：小型核仁RNA（snoRNA）是最早发现和研究最广泛的小型非编码RNA。但是，大多数研究集中在指导核糖体RNA修饰的snoRNA的一小部分。在这项研究中，我们注释了所有盒式C / D snoRNA在正常和癌细胞系中的表达模式，与其功能无关。结果表明，C / D snoRNAs以两种不同的形式表达，其末端相对于框C和D以及末端茎长度不同。两种形式均在癌细胞系中过表达，但显示出保守的末端分布。出人意料的是，长形式比短形式更依赖于核心snoRNP蛋白NOP58的表达，核心蛋白对于生产C / D snoRNA至关重要。相反，简短形式的子集取决于剪接因子RB-FOX2。对两种形式的潜在二级结构的分析表明，结合NOP58所需的k-turn基序在短形式下较不稳定，因此不太可能成熟为标准的snoRNP。数据合计表明，C / D snoRNAs至少分为两组，具有不同的成熟度和功能偏好。

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