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首页> 外文期刊>Nucleic Acids Research >Structure of a human pre-40S particle points to a role for RACK1 in the final steps of 18S rRNA processing
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Structure of a human pre-40S particle points to a role for RACK1 in the final steps of 18S rRNA processing

机译：人类40S之前颗粒的结构指出RACK1在18S rRNA加工的最终步骤中的作用

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摘要

Synthesis of ribosomal subunits in eukaryotes is a complex and tightly regulated process that has been mostly characterized in yeast. The discovery of a growing number of diseases linked to defects in ribosome biogenesis calls for a deeper understanding of these mechanisms and of the specificities of human ribosome maturation. We present the 19 resolution cryo-EM reconstruction of a cytoplasmic precursor to the human small ribosomal subunit, purified by using the tagged ribosome biogenesis factor LTV1 as bait. Compared to yeast pre-40S particles, this first three-dimensional structure of a human 40S subunit precursor shows noticeable differences with respect to the position of ribosome biogenesis factors and uncovers the early deposition of the ribosomal protein RACK1 during subunit maturation. Consistently, RACK1 is required for efficient processing of the 18S rRNA 3'-end, which might be related to its role in translation initiation. This first structural analysis of a human pre-ribosomal particle sets the grounds for high-resolution studies of conformational transitions accompanying ribosomal subunit maturation.
机译：真核生物中核糖体亚基的合成是一个复杂且受到严格控制的过程，大多数过程已在酵母中得到表征。与核糖体生物发生缺陷有关的越来越多的疾病的发现，要求人们对这些机制以及人类核糖体成熟的特异性有更深入的了解。我们提出了对人类小核糖体亚基的细胞质前体的19 <去除>分辨率的冷冻-EM重建，通过使用标记的核糖体生物发生因子LTV1作为诱饵进行纯化。与40S前酵母颗粒相比，人类40S亚基前体的第一个三维结构在核糖体生物发生因子的位置方面显示出明显差异，并揭示了亚基成熟过程中核糖体蛋白RACK1的早期沉积。一致地，RACK1是有效处理18S rRNA 3'-末端所必需的，这可能与其在翻译起始中的作用有关。人类核糖体前颗粒的首次结构分析为伴随核糖体亚基成熟的构象转变的高分辨率研究奠定了基础。

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