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首页> 外文期刊>RSC Advances >Nanofibrous rhPDGF-eluting PLGA-collagen hybrid scaffolds enhance healing of diabetic wounds
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Nanofibrous rhPDGF-eluting PLGA-collagen hybrid scaffolds enhance healing of diabetic wounds

机译：纳米纤维rhPDGF洗脱PLGA-胶原杂合支架可促进糖尿病伤口的愈合

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Patients with chronic, non-healing diabetic ulcers extend hospital stays and increase the financial burden more than non-diabetics. This investigation developed nanofibrous growth factor-eluting poly(lactic-co-glycolic acid) (PLGA)-collagen hybrid scaffolds that enabled the sustainable release of recombinant human platelet-derived growth factor (rhPDGF) to treat diabetic wounds. RhPDGF, collagen, and PLGA were dissolved in hexafluoroisopropanol, and then electrospun into nanofibrous scaffolds. An enzyme-linked immunosorbent assay kit and an elution method were utilized to evaluate the rates of in vivo and in vitro release of growth factors from the scaffolds. High concentrations and effectiveness of rhPDGF were documented for over three weeks. The water contact angles of nanofibrous rhPDGF-eluting PLGA-collagen hybrid scaffolds were less (97.2 +/- 0.7 degrees) than those of PLGA-collagen hybrid mesh (107.6 +/- 1.0 degrees) or virgin PLGA (113 +/- 3.3 degrees) (all post hoc p < 0.05). The nanofibers with rhPDGF-eluting PLGA-collagen hybrid scaffold also exhibited significantly higher water-retaining capacity than those in other groups (all post hoc p < 0.001). Furthermore, the scaffolds caused more re-epithelialization and contained more collagen I in rats with rhPDGF-eluting scaffolds than controls, as determined from the expressed matrix metalloproteinase 9 in the hair canals in developing hair follicles. These results revealed for the first time, the application of rhPDGF as a growth factor adjuvant, and developed a new collagen-based composite with potent cell infiltration and epithelialization properties.
机译：与非糖尿病患者相比，患有慢性非治愈性糖尿病溃疡的患者延长住院时间并增加经济负担。这项研究开发了洗脱纳米纤维生长因子的聚乳酸-乙醇酸（PLGA）-胶原蛋白混合支架，该支架能够可持续释放重组人血小板源性生长因子（rhPDGF），以治疗糖尿病伤口。将RhPDGF，胶原蛋白和PLGA溶解在六氟异丙醇中，然后电纺成纳米纤维支架。使用酶联免疫吸附测定试剂盒和洗脱方法来评估生长因子从支架中体内和体外释放的速率。三周内记录到高浓度和有效的rhPDGF。纳米纤维rhPDGF洗脱PLGA-胶原杂化支架的水接触角（97.2 +/- 0.7度）小于PLGA-胶原杂化网状支架（107.6 +/- 1.0度）或原生PLGA（113 +/- 3.3度） ）（所有事后p <0.05）。具有rhPDGF-洗脱的PLGA-胶原杂化支架的纳米纤维还显示出比其他组显着更高的保水能力（所有事后p <0.001）。此外，如从发育中的毛囊中的根管中表达的基质金属蛋白酶9所确定的，在用rhPDGF洗脱的大鼠中，该支架比在对照组中引起更多的上皮再生，并包含更多的胶原蛋白I。这些结果首次揭示了rhPDGF作为生长因子佐剂的应用，并开发了一种新型的基于胶原的复合材料，具有强大的细胞浸润和上皮形成特性。

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