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Secretion of metal-binding proteins by a newly discovered OsmY homolog in Cupriavidus metallidurans for the biogenic synthesis of metal nanoparticles

机译:新发现的OsmY同系物在金属古铜杯中分泌金属结合蛋白,用于金属纳米粒子的生物合成

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摘要

In this study, a well-characterized carrier, osmotically-inducible protein Y (OsmY), was employed in conjunction with a newly discovered protein homolog of Cupriavidus metallidurans for use in protein secretion. Rmet_4872 and Rmet_3428 were identified as possible homologs of OsmY based on sequence/feature identities. RFP fluorescence and immunoblotting analyses were used to identify and quantify the secretion of fusion proteins. Two metal-binding proteins were used to demonstrate the applications of protein secretion in biogenic synthesis of metal nanoparticles. Our study highlights the potential of applying protein secretion to mediate the reduction of metal ions.
机译:在这项研究中,特征明确的载体,渗透诱导的蛋白质Y(​​OsmY),与新发现的金属铜尿铜蛋白的蛋白质同源物一起用于蛋白质分泌。基于序列/特征同一性,Rmet_4872和Rmet_3428被确定为OsmY的可能同源物。 RFP荧光和免疫印迹分析用于鉴定和定量融合蛋白的分泌。两种金属结合蛋白被用来证明蛋白质分泌在金属纳米颗粒的生物合成中的应用。我们的研究突出了应用蛋白质分泌介导金属离子还原的潜力。

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