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首页> 外文期刊>RSC Advances >Development of an eco-friendly immunochromatographic test strip and its application in detecting Hg2+ without chelators
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Development of an eco-friendly immunochromatographic test strip and its application in detecting Hg2+ without chelators

机译：环保型免疫色谱试纸的研制及其在无螯合剂检测中的应用

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Here, a specific anti-Hg2(+) monoclonal antibody (mAb) was generated and an eco-friendly immunochromatographic test strip (EFITS) based on a mAb-nanogold probe for rapid and specific detection of Hg2(+) in water was developed. In the method, the conjugation of Hg-MNA (6-mercaptonicotinic acid)-BSA (bovine serum albumin) was synthesized as an immunogen, the conjugation of MNA-OVA (ovalbumin) was synthesized and selected as a coating antigen. The specific anti-Hg2(+) mAb from BALB/C female mice was screened based on a competitive immunoassay. The coating antigen and goat anti-mouse IgG antibody were coated on a nitrocellulose membrane (NC membrane) as a test line and a control line, respectively. The anti-Hg2(+) mAb-nanogold probe was applied to the conjugate pad. Hg2(+) competes with OVA-MNA for the mAb-nanogold probe causing a color change on the test line corresponding to the Hg2(+) content. Thus, we can distinguish the subtle differences through a strip reader. The resulting EFITS is able to detect Hg2(+) with LOD of 0.4 ng mL(-1) at 9 min by quantitative analysis. EFITS demonstrated here is eco-friendly (without Hg2(+) on the strip), capable of rapid detection, and does not require chelators.
机译：在这里，生成了特异性抗Hg2（+）单克隆抗体（mAb），并开发了一种基于mAb-纳米金探针的环保型免疫色谱测试条（EFITS），用于快速，特异性地检测水中的Hg2（+）。在该方法中，合成了Hg-MNA（6-巯基烟酸）-BSA（牛血清白蛋白）的结合物作为免疫原，合成了MNA-OVA（卵清蛋白）的结合物并选择作为包被抗原。基于竞争性免疫分析，筛选了来自BALB / C雌性小鼠的特异性抗Hg2（+）mAb。将包被抗原和山羊抗小鼠IgG抗体分别包被在硝酸纤维素膜（NC膜）上作为测试线和对照线。将抗Hg2（+）mAb-纳米金探针应用于偶联物垫。 Hg2（+）与OVA-MNA竞争mAb-纳米金探针，导致测试线上的颜色变化对应于Hg2（+）含量。因此，我们可以通过带状读取器来区分细微的差异。通过定量分析，所得的EFITS能够在9分钟内检测到LOD为0.4 ng mL（-1）的Hg2（+）。这里展示的EFITS是环保的（试纸上没有Hg2（+）），能够快速检测，并且不需要螯合剂。

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