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首页> 外文期刊>RSC Advances >Triple-helix molecular switch-induced hybridization chain reaction amplification for developing a universal and sensitive electrochemical aptasensor
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Triple-helix molecular switch-induced hybridization chain reaction amplification for developing a universal and sensitive electrochemical aptasensor

机译：三螺旋分子开关诱导的杂交链反应扩增，用于开发通用且灵敏的电化学适体传感器

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摘要

In this work, a universal and sensitive "signal-on" electrochemical aptasensor platform has been developed based on a triple-helix molecular switch (THMS)-induced hybridization chain reaction (HCR) amplification. This aptasensor platform system consists of a THMS-based molecular recognition process in a homogeneous solution and a HCR amplification on a gold electrode. In the absence of a target, the aptamer sequence is flanked by two arm segments (APT) and the triplex-forming oligonucleotide (TFO), forming a rigid THMS. It is in the eT off state. However, upon the introduction of a target, the interaction between the target and the APT leads to the dissociation of the THMS and thereby liberates the TFO, allowing the TFO to hybridize with the capture probe (CP) DNA and trigger the formation of dsDNA polymers through in situ HCR amplification. The dsDNA polymers cause the electrostatic attraction of numerous electroactive indicators [Ru(NH3)(6)](3+), resulting in significantly amplified electrochemical signal output. It is in the eT on state. As proof-of-principle, we use this approach to detect adenosine and human alpha-thrombin (Tmb), achieving lowest limit of detection values of 0.6 nM and 70.9 pM, respectively. As an electrochemical aptasensor platform, its universality can be easily realized by altering only the sequence of the APT, which provides a promising alternative to the electrochemical detection of a variety of analytes and may have potential applications in biomedical research and clinical diagnosis.
机译：在这项工作中，基于三螺旋分子开关（THMS）诱导的杂交链反应（HCR）扩增，开发了通用且灵敏的“信号接通”电化学适体传感器平台。该aptasensor平台系统由在均质溶液中基于THMS的分子识别过程和在金电极上的HCR扩增组成。在没有靶标的情况下，适体序列的侧翼是两个臂段（APT）和形成三链体的寡核苷酸（TFO），形成刚性的THMS。它处于eT关闭状态。然而，在引入靶标后，靶标与APT之间的相互作用导致THMS的解离，从而释放出TFO，从而使TFO与捕获探针（CP）DNA杂交并触发dsDNA聚合物的形成。通过原位HCR扩增。 dsDNA聚合物引起许多电活性指示剂[Ru（NH3）（6）]（3+）的静电吸引，从而导致电化学信号输出显着放大。它处于eT on状态。作为原理的证明，我们使用这种方法检测腺苷和人α凝血酶（Tmb），分别达到0.6 nM和70.9 pM的最低检测限。作为电化学适体传感器平台，只需更改APT的序列即可轻松实现其通用性，这为多种分析物的电化学检测提供了有希望的替代方法，并且在生物医学研究和临床诊断中可能具有潜在的应用。

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