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Antimicrobial, antioxidant and anticancer activity of biogenic silver nanoparticles - an experimental report

机译：生物银纳米颗粒的抗菌，抗氧化和抗癌活性-实验报告

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In the present study, use of a Helicteres isora stem bark extract for the biosynthesis of AgNPs is described. It was observed that the aqueous silver (Ag+) ions, once associated in the stem bark extract, were reduced in solution, thereby leading to formation of the stable AgNPs. These AgNPs were characterized using several techniques. The nanoparticles show a maximum absorbance at 419-431 nm in the ultraviolet-visible spectra. The presence of the steroid sapogenin was identified using Fourier transform-infrared (FTIR) spectroscopy. The reduction of the Ag+ ions to elemental silver was investigated by using X-ray diffraction (XRD). Transmission electron microscopy (TEM) revealed the formation of monodisperse, with low polydispersity, nanoparticles of 25.55 nm, and the presence of elemental silver was confirmed through energy dispersive spectroscopy (EDX) analysis. The AgNPs showed antioxidant activities such as DPPH, hydrogen peroxide and nitric oxide radical scavenging and a reducing power compared to the standard compounds. The antibacterial effect was determined against test strains, showing significant inhibition. The antiproliferative activity of the AgNPs was demonstrated using oral carcinoma (KB) cells with MTT, and confirmed using AO/EtBr, comet assay, DCFH-DA and Rhodamine 123 staining. In the toxicity study, a significant mortality rate was observed against Artemia with an IC50 concentration of 70 mu g mL(-1) and 108 h exposure. The NPs showed as cytotoxic against Artemia at 108 h, so they are cytotoxic at high concentrations and after prolonged exposure.
机译：在本研究中，描述了Helicteres isora茎皮提取物用于AgNPs的生物合成的用途。观察到，一旦在茎皮提取物中缔合，含水银（Ag +）离子在溶液中被还原，从而导致形成稳定的AgNPs。使用多种技术对这些AgNP进行了表征。纳米颗粒在紫外可见光谱中在419-431 nm处显示最大吸光度。使用傅立叶变换红外（FTIR）光谱法鉴定了类固醇皂甙元的存在。通过使用X射线衍射（XRD）研究了Ag +离子还原为元素银的过程。透射电子显微镜（TEM）显示形成了具有25.55 nm纳米颗粒的低分散性的单分散体，并且通过能量色散光谱（EDX）分析确认了元素银的存在。与标准化合物相比，AgNPs具有抗氧化活性，例如DPPH，过氧化氢和一氧化氮自由基清除能力和还原能力。确定了对测试菌株的抗菌作用，显示出明显的抑制作用。使用带有MTT的口腔癌细胞（KB）证明了AgNPs的抗增殖活性，并使用AO / EtBr，彗星分析，DCFH-DA和若丹明123染色证实了AgNPs的抗增殖活性。在毒性研究中，观察到针对Artemia的显着死亡率，IC50浓度为70μg mL（-1），暴露108 h。 NP在108 h表现出对卤虫的细胞毒性，因此它们在高浓度和长时间暴露后具有细胞毒性。

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