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Two-photon excited hemoglobin fluorescence provides contrast mechanism for label-free imaging of microvasculature in vivo

机译:双光子激发的血红蛋白荧光为体内微血管的无标记成像提供了对比机制

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摘要

Direct visualization of microvasculature provides significant insights in microcirculation and critically impacts the diagnosis and treatment of microcirculatory diseases. Recently, we discovered that the high-energy Soret fluorescence of hemoglobin peaked at 438 nm with an extremely short lifetime becomes strongly visible under two-photon excitation. Based on the distinct spectral and temporal characteristics of hemoglobin fluorescence, we demonstrated that two-photon fluorescence microscopy could become a powerful tool for label-free in vivo imaging of microvasculature in tissue.
机译:微血管的直接可视化提供了微循环方面的重要见识,并严重影响了微循环系统疾病的诊断和治疗。最近,我们发现,在双光子激发下,强烈可见到血红蛋白的高能Soret荧光峰在438 nm处达到极短的寿命。基于血红蛋白荧光的独特光谱和时间特征,我们证明了双光子荧光显微镜可以成为无标记体内微血管系统成像的有力工具。

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