Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Yan Zeng; Jin Xu; Dong Li; Li Li; Zilong Wen; Jianan Y. Qu

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Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

机译：斑马鱼中使用双光子自发荧光成像的无标记体内流式细胞术

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We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).

机译：我们展示了基于双光子激发自发荧光成像的斑马鱼血管中的无标签体内流式细胞仪。这项工作的主要发现是斑马鱼血液中血浆中强烈的自发荧光发射。血浆自发荧光为血管可视化和计数血细胞提供了出色的对比度。另外，细胞烟酰胺腺嘌呤二核苷酸自发荧光使白细胞（嗜中性粒细胞）的体内成像和计数成为可能。

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《Optics Letters》 |2012年第13期|共3页
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Yan Zeng; Jin Xu; Dong Li; Li Li; Zilong Wen; Jianan Y. Qu;
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1. Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging [J] . Yan Zeng, Jin Xu, Dong Li, Optics Letters . 2012,第13期

机译：斑马鱼中使用双光子自发荧光成像的无标记体内流式细胞术
2. In vivo label-free two-photon excitation autofluorescence microscopy of microvasculature using a 520 nm femtosecond fiber laser [J] . Optics Letters . 2020,第10期

机译：使用520 nm Femtosecond光纤激光器在体内无标记的双光子激发自动荧光显微镜显微镜显微镜
3. Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging: Application to in Vivo Imaging of Amyloid-β Plaques with a Negligible Background Signal [J] . Dokyoung Kim, Hyunsoo Moon, Sung Hoon Baik, Journal of the American Chemical Society . 2015,第21期

机译：具有最小自发荧光的双光子吸收染料在组织成像中的应用：在具有可忽略背景信号的淀粉样β斑块的体内成像中的应用
4. In vivo imaging flow cytometry based on laser scanning two-photon microscopy at kHz cross-sectional frame rate [C] . Lingjie Kong, Jianyong Tang, Meng Cui Multiphoton microscopy in the biomedical sciences XVI . 2016

机译：基于激光扫描双光子显微镜在kHz截面帧速率下的体内成像流式细胞术
5. Quantitative two-photon flow cytometry in vitro and in vivo. [D] . Zhong, Cheng. 2006

机译：体外和体内定量双光子流式细胞术。
6. In vivo micro-vascular imaging and flow cytometry in zebrafish using two-photon excited endogenous fluorescence [O] . Yan Zeng, Bo Yan, Qiqi Sun, 2014

机译：斑马鱼体内微血管成像和流式细胞仪使用双光子激发的内源荧光
7. In vivo micro-vascular imaging and flow cytometry in zebrafish using two-photon excited endogenous fluorescence [O] . Zeng, Yan, Yan, Bo, Sun, Qiqi, 2014

机译：斑马鱼体内微血管成像和流式细胞仪使用双光子激发的内源荧光

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

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