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Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians

机译:模仿人角膜内皮的光学镶嵌物的制造,用于培训和评估眼库技术人员

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摘要

The determination of endothelial cell density (ECD) is a crucial activity in eye banks for the assessment of corneal tissue quality. These cells are responsible for corneal transparency, and ECD correlates with graft survival. ECD is mainly assessed with a manual "naked-eye" procedure under a transmitted light microscope in Europe and using a specular microscope in the United States. Interbank and intrabank variability has been previously demonstrated. In order to facilitate training and continuing education of technicians and reliability assessment of eye banks' ECD determination, we use micro-optics technologies to fabricate test mosaics that exactly reproduce the image of human corneal endothelium. The description of the fabrication process is detailed, and comparisons are made between amplitude and phase mosaics.
机译:内皮细胞密度(ECD)的确定是眼库中评估角膜组织质量的关键活动。这些细胞负责角膜透明,ECD与移植物存活相关。在欧洲,ECD主要通过手动的“裸眼”程序在透射光显微镜下进行评估,而在美国使用镜面显微镜进行评估。以前已经证明了银行间和银行间的可变性。为了促进对技术人员的培训和继续教育以及对眼库ECD测定的可靠性评估,我们使用微光学技术制作了可以精确再现人角膜内皮图像的测试镶嵌图。详细描述了制造过程,并对幅度和相位镶嵌进行了比较。

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