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首页> 外文期刊>Optics Letters >Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures
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Motility-, autocorrelation-, and polarization-sensitive optical coherence tomography discriminates cells and gold nanorods within 3D tissue cultures

机译:运动,自相关和偏振敏感的光学相干断层扫描可区分3D组织培养物中的细胞和金纳米棒

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摘要

We propose a method for differentiating classes of light scatterers based upon their temporal and polarization properties computed from time series of polarization-sensitive optical coherence tomography (PS-OCT) images. The amplitude (motility) and time scale (autocorrelation decay time) of the speckle fluctuations are combined with the cross-polarization pixel-wise to render Motility-, autocorrelation-, and polarization-sensitive (MAPS) OCT contrast images. This combination of metrics provides high specificity for discriminating diffusive gold nano-rods and mammary epithelial cell spheroids within 3D tissue culture, based on their unique MAPS signature. This has implications toward highly specific contrast in molecular (nanoparticle-based) and functional (cellular activity) imaging using standard PS-OCT hardware.
机译:我们提出了一种根据光散射体的时间和偏振特性区分光散射体类别的方法,这些特性是根据偏振敏感光学相干层析成像(PS-OCT)图像的时间序列计算得出的。斑点波动的幅度(运动性)和时间标度(自相关衰减时间)与像素交叉极化相结合,以提供运动性,自相关性和偏振敏感(MAPS)OCT对比图像。度量标准的这种组合基于其独特的MAPS签名,为区分3D组织培养物中的扩散性金纳米棒和乳腺上皮细胞球体提供了高特异性。这暗示了使用标准PS-OCT硬件在分子(基于纳米粒子)和功能(细胞活性)成像中的高特异性对比。

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