Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

Doronina-Amitonova L. V.; Fedotov I. V.; Zheltikov A. M.

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Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

机译：通过时间调制的受激发射损耗进行超高对比度成像

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Stimulated emission depletion (STED) is the key optical technology enabling super-resolution microscopy below the diffraction limit. Here, we demonstrate that modulation of STED in the time domain, combined with properly designed lock-in detection, can radically enhance the contrast of fluorescent images of strongly autofluorescent biotissues. In our experiments, the temporally modulated STED technique, implemented with low-intensity continuous- wave laser sources, is shown to provide an efficient all-optical suppression of a broadband fluorescent background, allowing the contrast of fluorescent images of mammal brain tissues tagged with nitrogen-vacancy diamond to be increased by five orders of magnitude. (C) 2015 Optical Society of America

机译：受激发射损耗（STED）是关键光学技术，可在低于衍射极限的条件下进行超分辨率显微镜检查。在这里，我们证明了在时域中对STED的调制与适当设计的锁定检测相结合，可以从根本上增强强自发荧光生物组织的荧光图像的对比度。在我们的实验中，采用低强度连续波激光源实施的时域调制STED技术显示出对宽带荧光背景的有效全光抑制，从而可以对比标记有氮的哺乳动物脑组织的荧光图像。空缺钻石将增加五个数量级。（C）2015年美国眼镜学会

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《Optics Letters》 |2015年第5期|共4页
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Doronina-Amitonova L. V.; Fedotov I. V.; Zheltikov A. M.;
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1. Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion [J] . Doronina-Amitonova L. V., Fedotov I. V., Zheltikov A. M. Optics Letters . 2015,第5期

机译：通过时间调制的受激发射损耗进行超高对比度成像
2. A montage of images through the division septum of E. coli E. coli cells. The images were captured by dual‐color time‐gated Stimulated Emission Depletion (STED) nanoscopy, on cells simultaneously expressing FtsZ‐mNeonGreen (green) and mStrawberry‐FtsN (red). The images show that FtsZ and FtsN are spatially separated. For details, see the article by S?derstr?m et al. on pp. 387‐401 of this issue. [J] . Molecular Microbiology . 2018,第3期

机译：通过大肠杆菌大肠杆菌细胞的分区隔膜的图像蒙太奇。通过双色时间门控刺激发射耗尽（STED）纳米镜，在同时表达FTSZ-Mneongreen（绿色）和Mstrawberry-FTSN（红色）的细胞上捕获图像。图像显示FTSZ和FTSN在空间上分开。有关详细信息，请参阅S？Derstr？M等人。在第387-401页上。
3. Dual-mode super-resolution imaging with stimulated emission depletion microscopy and fluorescence emission difference microscopy [J] . Wang Yifan, Ma Ye, Kuang Cuifang, Applied optics . 2015,第17期

机译：双模超分辨率成像，具有激发发射耗尽显微镜和荧光发射差异显微镜
4. Temporal characteristic simulation for stimulated emission depletion microscopy [C] . Wenxia Chen, Fanrong Xiao, Li Liu, International Symposium on Photonics, Biophotonics, and Nanophotonics'05 . 2005

机译：瞬态特性模拟的受激发射损耗显微镜
5. Development and application of two-photon excitation stimulated emission depletion microscopy for superresolution fluorescence imaging in thick tissue [D] . Takasaki, Kevin T. 2013

机译：厚组织中超分辨率荧光成像的双光子激发激发发射耗尽显微镜的开发与应用
6. All-optical fluorescence image recovery using modulated Stimulated Emission Depletion [O] . Chaoyang Fan, Jung-Cheng Hsiang, Amy E. Jablonski, -1

机译：全光荧光图像恢复使用调制受激发射损耗
7. All-optical fluorescence image recovery using modulated stimulated emission depletion [O] . Chaoyang Fan, Jung-Cheng Hsiang, Amy E. Jablonski, 2011

机译：全光学荧光图像恢复使用调制的刺激排放耗尽

Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

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