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首页> 外文期刊>Physical chemistry chemical physics: PCCP >Conformer-selective photoelectron spectroscopy of α-lactalbumin derived multianions in the gas phase
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Conformer-selective photoelectron spectroscopy of α-lactalbumin derived multianions in the gas phase

机译：气相中α-乳白蛋白衍生的多阴离子的共形物选择光电子能谱

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We have recorded conformer-selective, gas-phase photoelectron spectra of α-lactalbumin derived multianions generated by electrospraying solutions of both the native protein and its denatured form (as prepared by breaking the sulfur-sulfur bonds by chemical reduction). Three different groups of gas-phase multianion conformers have been observed and characterized. Highly-folded and partially-unfolded structures are obtained from solutions of the native protein. Only highly-elongated conformers are observed upon electrospraying the denatured protein. Adiabatic detachment energies were determined at several negative charge states for each conformer group. In comparison to highly-elongated conformations, highly-folded structures show a steeper decrease of electron binding energy with increasing negative charge. By comparing experimental detachment energies for highly-elongated structures with the predictions of a simple electrostatic model calculation, we have determined the effective dielectric shielding constant.
机译：我们已经记录了通过电喷雾天然蛋白质及其变性形式（通过化学还原破坏硫-硫键制得的溶液）生成的α-乳白蛋白衍生的多阴离子的构象选择性气相光电子光谱。已经观察和表征了三组不同的气相多阴离子构象异构体。高度折叠和部分展开的结构是从天然蛋白质溶液中获得的。电喷雾变性蛋白后，仅观察到高度延伸的构象体。对于每个构象基团，在几个负电荷状态下确定了绝热脱离能。与高度伸长的构象相比，高度折叠的结构显示出随着负电荷增加，电子结合能急剧下降。通过将高度伸长的结构的实验脱离能与简单静电模型计算的预测进行比较，我们确定了有效的介电屏蔽常数。

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