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首页> 外文期刊>Cornea >Alterations of extracellular matrix components and proteinases in human corneal buttons with INTACS for post-laser in situ keratomileusis keratectasia and keratoconus.
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Alterations of extracellular matrix components and proteinases in human corneal buttons with INTACS for post-laser in situ keratomileusis keratectasia and keratoconus.

机译：激光后原位角膜磨镶术和圆锥角膜的INTACS对人角膜纽扣中细胞外基质成分和蛋白酶的改变。

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摘要

PURPOSE: To perform an immunohistochemical evaluation of corneas with INTACS for post-laser in situ keratomileusis (LASIK) keratectasia and keratoconus, obtained after corneal transplantation. METHODS: Corneas from 1 patient with INTACS for post-LASIK keratectasia and 2 patients with INTACS for keratoconus were obtained within 3 hours after penetrating keratoplasty, and cryostat sections were analyzed by immunostaining for 35 extracellular matrix (ECM) components and proteinases. RESULTS: In the stroma of all corneas next to an INTACS implant, ECM components typically associated with fibrosis were observed. These included tenascin-C, fibrillin-1, and types III, IV (alpha1/alpha2 chains), and XIV collagen. Also, significant deposition of perlecan, nidogen-2, and cellular fibronectin was revealed in the same locations. The keratoconus cases displayed typical Bowman layer breaks and subepithelial fibrosis with deposition of various ECM components. In all cases, some keratocytes around INTACS were positivefor specific proteinases associated with stromal remodeling, including cathepsins F and H, matrix metalloproteinase (MMP)-1, MMP-3, and MMP-10. Staining for MMP-7 was variable; MMP-2 and MMP-9 were mostly negative. Patterns of type IV collagen alpha 3, alpha 4, and alpha 6 chains; types VI and VIII collagen; laminin-332, alpha 4, alpha 5, beta1, beta2, and gamma 1 laminin chains; vitronectin; thrombospondin-1; urokinase; EMMPRIN; and cathepsins B and L were unchanged around INTACS in all 3 cases compared with normal. CONCLUSIONS: Abnormal accumulation of fibrotic ECM components and proteinases near INTACS suggests ongoing lysis and remodeling of corneal stroma. Specific changes observed in each case may be related to underlying pathology.
机译：目的：用INTACS对角膜移植后获得的激光原位角膜磨镶术（LASIK）角膜扩张和圆锥角膜进行角膜的免疫组织化学评估。方法：在穿透性角膜移植术后3小时内，从1例LASIK术后角化病的INTACS患者和2例圆锥角膜的INTACS患者中获取角膜，并通过免疫染色分析35个细胞外基质（ECM）成分和蛋白酶的低温恒温器切片。结果：在INTACS植入物旁边的所有角膜基质中，观察到通常与纤维化相关的ECM成分。这些包括腱糖蛋白C，原纤维蛋白1和III，IV型（alpha1 / alpha2链）和XIV胶原蛋白。同样，在相同的位置还发现了perlecan，nidogen-2和细胞纤连蛋白的大量沉积。圆锥角膜病例表现出典型的鲍曼层断裂和上皮下纤维化，伴有各种ECM成分沉积。在所有情况下，INTACS周围的某些角膜细胞对与基质重塑相关的特定蛋白酶呈阳性，包括组织蛋白酶F和H，基质金属蛋白酶（MMP）-1，MMP-3和MMP-10。 MMP-7的染色是可变的。 MMP-2和MMP-9大多为阴性。 IV型胶原蛋白alpha 3，alpha 4和alpha 6链的模式; VI型和VIII型胶原; Laminin-332，α4，α5，beta1，beta2和gamma 1层粘连蛋白链;玻璃连蛋白血小板反应蛋白-1;尿激酶EMMPRIN;与正常相比，所有3例INTACS周围的组织蛋白酶B和L均未发生变化。结论：INTACS附近纤维化ECM成分和蛋白酶的异常积累表明角膜基质的持续溶解和重塑。在每种情况下观察到的特定变化可能与潜在的病理学有关。

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